To bind plasmid DNA to the silica matrix, the DNA has to be suspended in a chaotropic agent and high salt. This statement is: a. True. b. False. A chaotropic agent removes the monomolecular layer of water molecules attached to the silica membrane, making it possible for DNA to bind to the silica membrane. a. True. b. False 7. The third and fourth-generation DNA sequencing methods do not require shearing DNA molecules into short fragments. a. True. b. False. The annealing reaction's nucleation step is unnecessary because both single- strand plasmid DNA circles are held together. Thus, plasmid DNA reannealing proceeds only by the zippering reaction. a. True. b. False

Human Heredity: Principles and Issues (MindTap Course List)
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Chapter13: An Introduction To Genetic Technology
Section: Chapter Questions
Problem 11QP: Cloning Genes Is a Multistep Process In cloning human DNA, why is it necessary to insert the DNA...
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The following True/False questions all relate to DNA.

To bind plasmid DNA to the silica matrix, the DNA has to be suspended in a
chaotropic agent and high salt. This statement is:
a. True.
b. False.
A chaotropic agent removes the monomolecular layer of water molecules
attached to the silica membrane, making it possible for DNA to bind to the
silica membrane.
a. True.
b. False
7. The third and fourth-generation DNA sequencing methods do not require
shearing DNA molecules into short fragments.
a. True.
b.
False.
5. The annealing reaction's nucleation step is unnecessary because both single-
strand plasmid DNA circles are held together. Thus, plasmid DNA
reannealing proceeds only by the zippering reaction.
a. True.
b. False
Transcribed Image Text:To bind plasmid DNA to the silica matrix, the DNA has to be suspended in a chaotropic agent and high salt. This statement is: a. True. b. False. A chaotropic agent removes the monomolecular layer of water molecules attached to the silica membrane, making it possible for DNA to bind to the silica membrane. a. True. b. False 7. The third and fourth-generation DNA sequencing methods do not require shearing DNA molecules into short fragments. a. True. b. False. 5. The annealing reaction's nucleation step is unnecessary because both single- strand plasmid DNA circles are held together. Thus, plasmid DNA reannealing proceeds only by the zippering reaction. a. True. b. False
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