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You extract DNA from 200 milligram of wheat germ. Your total volume of DNA extraction sample is 500 microlitres. You pipette 200 microlitres of this extraction sample into a 3-ml cuvette and make up to 3.0 ml using buffer. The absorbance at 260 nm (A260) of the solution in the cuvette is 0.2 (Remember: an A260 of 1.0 corresponds to a DNA concentration in the cuvette solution of 50 micrograms per ml). Calculate the total amount of DNA in 100g wheat germ, assuming that the extraction efficiency of DNA from wheat germ during your lab protocol is 40%. Please fill out each of the boxes in the Table below What to Calculate Calculation / Result DNA conconti

You extract DNA from 200 milligram of wheat germ. Your total volume of DNA extraction sample is 500 microlitres. You pipette 200 microlitres of this extraction sample into a 3-ml cuvette and make up to 3.0 ml using buffer. The absorbance at 260 nm (A260) of the solution in the cuvette is 0.2 (Remember: an A260 of 1.0 corresponds to a DNA concentration in the cuvette solution of 50 micrograms per ml). Calculate the total amount of DNA in 100g wheat germ, assuming that the extraction efficiency of DNA from wheat germ during your lab protocol is 40%. Please fill out each of the boxes in the Table below What to Calculate Calculation / Result DNA conconti

Biochemistry
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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You extract DNA from 200 milligram of wheat germ. Your total volume of DNA extraction sample is 500 microlitres. You pipette 200 microlitres of this extraction sample into a 3-ml cuvette and make up to 3.0 ml using buffer. The absorbance at 260 nm (A260) of the solution in the cuvette is 0.2 (Remember: an A260 of 1.0 corresponds to a DNA concentration in the cuvette solution of 50 micrograms per ml). Calculate the total amount of DNA in 100g wheat germ, assuming that the extraction efficiency of DNA from wheat germ during your lab protocol is 40%. Please fill out each of the boxes in the Table below What to Calculate Calculation / Result DNA concentration in cuvette solution (in microgram per ml) Dilution factor of DNA sample in cuvette Total amount (microgram) of DNA in the entire extraction sample Total amount (milligram) of DNA in 100g wheat germ assuming an extraction efficiency of 40%
Transcribed Image Text:You extract DNA from 200 milligram of wheat germ. Your total volume of DNA extraction sample is 500 microlitres. You pipette 200 microlitres of this extraction sample into a 3-ml cuvette and make up to 3.0 ml using buffer. The absorbance at 260 nm (A260) of the solution in the cuvette is 0.2 (Remember: an A260 of 1.0 corresponds to a DNA concentration in the cuvette solution of 50 micrograms per ml). Calculate the total amount of DNA in 100g wheat germ, assuming that the extraction efficiency of DNA from wheat germ during your lab protocol is 40%. Please fill out each of the boxes in the Table below What to Calculate Calculation / Result DNA concentration in cuvette solution (in microgram per ml) Dilution factor of DNA sample in cuvette Total amount (microgram) of DNA in the entire extraction sample Total amount (milligram) of DNA in 100g wheat germ assuming an extraction efficiency of 40%
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