In addition, the proportion of each electron carrier (ferredoxin, NADP+, plastoquinone, cyt b559, cyt f, plastocyanin and cyt b6) that is oxidized during photosynthesis can be measured spectrophotometrically. From the data given below, identify each column with one of the electron carriers listed above, and give a short reason to justify each choice. One of the electron carriers might not be represented by a letter. There may be ambiguity, where you can’t narrow down one letter between two substances – that’s fine, just explain why you can’t tell.   The number shows the proportion of the lettered electron carrier that is oxidized; that is 0.0 means none of that carrier is oxidized, and 1.0 means that it is fully oxidized.   (attached table) A = B = C = D = E = F =

Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
icon
Related questions
Question
In addition, the proportion of each electron carrier (ferredoxin, NADP+,
plastoquinone, cyt b559, cyt f, plastocyanin and cyt b6) that is oxidized during
photosynthesis can be measured spectrophotometrically. From the data given below,
identify each column with one of the electron carriers listed above, and give a short
reason to justify each choice. One of the electron carriers might not be represented by a
letter. There may be ambiguity, where you can’t narrow down one letter between two
substances – that’s fine, just explain why you can’t tell.
 
The number shows the proportion of the lettered electron carrier that is oxidized; that is
0.0 means none of that carrier is oxidized, and 1.0 means that it is fully oxidized.
 
(attached table)

A =
B =
C =
D =
E =
F =
 
 
Inhibitor added
none
methylviologen
Ferricyanide
DCMU
Phenazine
methosulfate
desaspidin
A
0.75
0.75
0.75
0.75
0.75
0.75
B
0.5
0.5
0.5
1.0
0.5
0.5
C
0.5
1.0
1.0
0.5
0.5
0.5
D
0.5
0.5
0.5
0.5
0.5
0.5
E
0.25
0.25
0.25
1.0
0.25
0.25
F
0.5
1.0
1.0
0.5
1.0
0.5
Transcribed Image Text:Inhibitor added none methylviologen Ferricyanide DCMU Phenazine methosulfate desaspidin A 0.75 0.75 0.75 0.75 0.75 0.75 B 0.5 0.5 0.5 1.0 0.5 0.5 C 0.5 1.0 1.0 0.5 0.5 0.5 D 0.5 0.5 0.5 0.5 0.5 0.5 E 0.25 0.25 0.25 1.0 0.25 0.25 F 0.5 1.0 1.0 0.5 1.0 0.5
Objective: To reconstruct some of the experiments that were performed on
Photosystems I and II in order to determine whether they were coupled, and then
determine in what sequence the various electron carriers were used in transport.
1. Use the photosystem I and II diagram below, which is similar but not identical to
the one in the text. As in oxidative phosphorylation, in which high-energy electrons are
transferred during the production of stored chemical energy, photosynthesis can be
inhibited at different points within the system by a variety of chemicals.
Ferredoxin (F)
P700
cyt b6
●
plastocyanin (PC)
NADPH
NADP+ + H+
cyt f
ADP + Pi
ATP
cyt b559
DCMU prevents oxidation of water
Methylviologen accepts electrons from P700
H₂O
Plastiquinone (Q)
1/2 O₂ + 2 H+
P680
• Desaspidin uncouples phosphorylation
Ferricyanide accepts electrons from the end of the transport chain
ATP
ADP + P₁
Phenazine methosulfate accepts electrons from ferrodoxin and donates the electrons
directly to the transport chain
Transcribed Image Text:Objective: To reconstruct some of the experiments that were performed on Photosystems I and II in order to determine whether they were coupled, and then determine in what sequence the various electron carriers were used in transport. 1. Use the photosystem I and II diagram below, which is similar but not identical to the one in the text. As in oxidative phosphorylation, in which high-energy electrons are transferred during the production of stored chemical energy, photosynthesis can be inhibited at different points within the system by a variety of chemicals. Ferredoxin (F) P700 cyt b6 ● plastocyanin (PC) NADPH NADP+ + H+ cyt f ADP + Pi ATP cyt b559 DCMU prevents oxidation of water Methylviologen accepts electrons from P700 H₂O Plastiquinone (Q) 1/2 O₂ + 2 H+ P680 • Desaspidin uncouples phosphorylation Ferricyanide accepts electrons from the end of the transport chain ATP ADP + P₁ Phenazine methosulfate accepts electrons from ferrodoxin and donates the electrons directly to the transport chain
Expert Solution
steps

Step by step

Solved in 3 steps with 2 images

Blurred answer
Similar questions
  • SEE MORE QUESTIONS
Recommended textbooks for you
Biochemistry
Biochemistry
Biochemistry
ISBN:
9781319114671
Author:
Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:
W. H. Freeman
Lehninger Principles of Biochemistry
Lehninger Principles of Biochemistry
Biochemistry
ISBN:
9781464126116
Author:
David L. Nelson, Michael M. Cox
Publisher:
W. H. Freeman
Fundamentals of Biochemistry: Life at the Molecul…
Fundamentals of Biochemistry: Life at the Molecul…
Biochemistry
ISBN:
9781118918401
Author:
Donald Voet, Judith G. Voet, Charlotte W. Pratt
Publisher:
WILEY
Biochemistry
Biochemistry
Biochemistry
ISBN:
9781305961135
Author:
Mary K. Campbell, Shawn O. Farrell, Owen M. McDougal
Publisher:
Cengage Learning
Biochemistry
Biochemistry
Biochemistry
ISBN:
9781305577206
Author:
Reginald H. Garrett, Charles M. Grisham
Publisher:
Cengage Learning
Fundamentals of General, Organic, and Biological …
Fundamentals of General, Organic, and Biological …
Biochemistry
ISBN:
9780134015187
Author:
John E. McMurry, David S. Ballantine, Carl A. Hoeger, Virginia E. Peterson
Publisher:
PEARSON