If the above gene is one of the three structural genes of the lac operon that codes for the protein/ enzyme responsible for breaking lactose into two molecules of simple sugars, a.) what triggers the activation of this gene? b.) what triggers the inactivation of this gene? c.) what substance is attached to the operator region of the operon in the absence of activator? d.) what gene is responsible for the synthesis of the substance used to attach in the operator region in the absence of activator? e.) what substance is attached to the promoter region of the operon in this case?

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1. If the above gene is one of the three structural genes of the lac operon that codes for the
protein/ enzyme responsible for breaking lactose into two molecules of simple sugars,
a.) what triggers the activation of this gene?
b.) what triggers the inactivation of this gene?
c.) what substance is attached to the operator region of the operon in the absence of
activator?
d.) what gene is responsible for the synthesis of the substance used to attach in the
operator region in the absence of activator?

e.) what substance is attached to the promoter region of the operon in this case? 

N.B.: Transcription proceeds in 5'-3' on the DNA template. Ribosomes move in 5'-3' along
the mRNA beginning in 5’AUG3' codon.
PROCEDURE
Below is segment of a DNA coding strand (5'-3') with functional gene highlighted in yellow:
1261 tcgcaactct ctactgtttc tccatacccg tttttttggg ctagaaataa ttttgtttaa
1321 ctttaagaag gagatataca tatggctago aaaggagaag aacttttcac tggagttgtc
1381 ccaattcttg ttgaattaga tggtgatgtt aatgggcaca aattttctgt cagtggagag
1441 ggtgaaggtg atgctacata cggaaagctt acccttaaat ttatttgcac tactggaaaa
1501 ctacctgttc catggccaac acttgtcact actttctctt atggtgttca atgcttttcc
1561 cgttatccgg atcatatgaa acggcatgac tttttcaaga gtgccatgcc cgaaggttat
1621 gtacaggaac gcactatatc tttcaaagat gacgggaact acaagacgcg tgctgaagtc
1681 aagtttgaag gtgataccct tgttaatcgt atcgagttaa aaggtattga ttttaaagaa
1
1741 gatggaaaca ttctcggaca caaactcgag tacaactata actcacacaa tgtatacatc
1801 acggcagaca aacaaaagaa tggaatcaaa gctaacttca aaattcgcca caacattgaa
1861 gatggatccg ttcaactago agaccattat caacaaaata ctccaattgg cgatggccct
1921 gtccttttac cagacaacca ttacctgtcg acacaatctg ccctttcgaa agatcccaac
1981 gaaaagcgtg accacatggt ccttcttgag tttgtaactg ctgctgggat tacacatggc
2041 atggatgagc tctacaaata atgaattega gctcggtacc cggggatcct ctagagtcga
2101 cctgcaggca tgcaagcttg gctgttttgg cggatgagag aagattttca gcctgataca
Transcribed Image Text:N.B.: Transcription proceeds in 5'-3' on the DNA template. Ribosomes move in 5'-3' along the mRNA beginning in 5’AUG3' codon. PROCEDURE Below is segment of a DNA coding strand (5'-3') with functional gene highlighted in yellow: 1261 tcgcaactct ctactgtttc tccatacccg tttttttggg ctagaaataa ttttgtttaa 1321 ctttaagaag gagatataca tatggctago aaaggagaag aacttttcac tggagttgtc 1381 ccaattcttg ttgaattaga tggtgatgtt aatgggcaca aattttctgt cagtggagag 1441 ggtgaaggtg atgctacata cggaaagctt acccttaaat ttatttgcac tactggaaaa 1501 ctacctgttc catggccaac acttgtcact actttctctt atggtgttca atgcttttcc 1561 cgttatccgg atcatatgaa acggcatgac tttttcaaga gtgccatgcc cgaaggttat 1621 gtacaggaac gcactatatc tttcaaagat gacgggaact acaagacgcg tgctgaagtc 1681 aagtttgaag gtgataccct tgttaatcgt atcgagttaa aaggtattga ttttaaagaa 1 1741 gatggaaaca ttctcggaca caaactcgag tacaactata actcacacaa tgtatacatc 1801 acggcagaca aacaaaagaa tggaatcaaa gctaacttca aaattcgcca caacattgaa 1861 gatggatccg ttcaactago agaccattat caacaaaata ctccaattgg cgatggccct 1921 gtccttttac cagacaacca ttacctgtcg acacaatctg ccctttcgaa agatcccaac 1981 gaaaagcgtg accacatggt ccttcttgag tttgtaactg ctgctgggat tacacatggc 2041 atggatgagc tctacaaata atgaattega gctcggtacc cggggatcct ctagagtcga 2101 cctgcaggca tgcaagcttg gctgttttgg cggatgagag aagattttca gcctgataca
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