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a) In an experiment, you have changed a DNA sequence directly upstream from a start codon of an operon in E.coli. The reason is to investigate the function of this region of the DNA. The analysis revealed that after the changes, the same amount of mRNA is made from the operon, but very few proteins are being produced. What is the most likely DNA region that you have changed, and what is the function of this region? b) The lacZ gene, which encodes for the expression of ß-galactosidase during lactose metabolism, depends very much upon the presence or absence of lactose in the environment. In an experiment, you have introduced cellobiose, an analogue of lactose which also acts as an inhibitor of the enzyme, to study its effect on B-galactosidase activity. What do you foresee to observe, in relation to lactose metabolism?

a) In an experiment, you have changed a DNA sequence directly upstream from a start codon of an operon in E.coli. The reason is to investigate the function of this region of the DNA. The analysis revealed that after the changes, the same amount of mRNA is made from the operon, but very few proteins are being produced. What is the most likely DNA region that you have changed, and what is the function of this region? b) The lacZ gene, which encodes for the expression of ß-galactosidase during lactose metabolism, depends very much upon the presence or absence of lactose in the environment. In an experiment, you have introduced cellobiose, an analogue of lactose which also acts as an inhibitor of the enzyme, to study its effect on B-galactosidase activity. What do you foresee to observe, in relation to lactose metabolism?

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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a) In an experiment, you have changed a DNA sequence directly upstream from a start codon of an operon in E.coli. The reason is to investigate the function of this region of the DNA. The analysis revealed that after the changes, the same amount of mRNA is made from the operon, but very few proteins are being produced. What is the most likely DNA region that you have changed, and what is the function of this region? b) The lacZ gene, which encodes for the expression of B-galactosidase during lactose metabolism, depends very much upon the presence or absence of lactose in the environment. In an experiment, you have introduced cellobiose, an analogue of lactose which also acts as an inhibitor of the enzyme, to study its effect on B-galactosidase activity. What do you foresee to observe, in relation to lactose metabolism?
Transcribed Image Text:a) In an experiment, you have changed a DNA sequence directly upstream from a start codon of an operon in E.coli. The reason is to investigate the function of this region of the DNA. The analysis revealed that after the changes, the same amount of mRNA is made from the operon, but very few proteins are being produced. What is the most likely DNA region that you have changed, and what is the function of this region? b) The lacZ gene, which encodes for the expression of B-galactosidase during lactose metabolism, depends very much upon the presence or absence of lactose in the environment. In an experiment, you have introduced cellobiose, an analogue of lactose which also acts as an inhibitor of the enzyme, to study its effect on B-galactosidase activity. What do you foresee to observe, in relation to lactose metabolism?
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