1. A. A mutation in E. coli is found that fails to induce the arabinose operon. Upon further investigation, it is found that this mutant also is unable to induce the lac operon. Which of the following single mutations could explain this phenotype (you may choose one or more answers a) Mutation in the promoter of the ara operon b) Mutation in the promoter of the lac operon c) Mutation in the operator of the lac operon d) Mutation in the AraC protein for the ara operon e) Mutation in the repressor of the lac operon f) Mutation in the Y gene for Lac permease g) Mutation in the gene for adenylate cyclase h) Mutation in the gene for CAP (or CRP) protein i) Mutation in the gene for rho factor j) None of the above

Q: The map of the lac operon is shown below. Consider the following examples that include both haploids…

A: Lac operon is the segment of DNA which includes regulatory gene, structural gene, promoter gene and…

Q: 1) The logic of the Lac operon was deciphered by using bacterial lac-operon mutants. Crucial to the…

A: Lac operon is a genetic unit in bacteria that helps in the metabolism of lactose in E.coli and lac…

Q: Assuming that the level of glucose is low, a mutation in the repressor associated with the lac…

A: The lac operon stands for lactose operon. These are a set of genes that are found in E.coli and…

Q: closely linked open reading frames (ORFs) have been identified on the E. coli chromosome and they…

A: E.coli being a prokaryote consists of an operon which is the cluster of genes that helps in the…

Q: Operons are a common feature of bacterial genomes. Which of the following statements about the lac…

A: Lac operon has three important genes that helps the bacteria to take up the lactose sugar when…

Q: For the following questions, describe the expression levels of the structural genes in the Trp…

A: Tryptophan is an essential amino acid, and the trp operon is a genetic regulatory system that can be…

Q: If lactose isn't present in the environment of an E. coli, what is the state of the lac operon? O a.…

A: The lactose operon (also known as the lac operon) is a group of genes present in E. coli and other…

Q: The trp operon in E. coli is a negative repressible operon. This implies that...   a. The trp…

A: The correct answer is:c. The trp operon makes use of a repressor and can be turned "on" (i.e., is…

Q: he lac operon contains the genes that the bacterium needs to be able to use lactose as a carbon…

A: An Operon is a unit of structure and function that consists of the clusters of regulatory genes…

Q: Which of the following statements about the trp operon is false?

A: From above question the statement  If a mutation in region 2 prevents the formation of the 2 + 3…

Q: Predict the effect on the activity of the lac operon of a mutation that disrupts the function of: a)…

A: The Catabolic Activator Protein (CAP) is a protein which acts as a positive regulating factor for…

Q: coli WT (wild type, everything is functional) and II) E. coli whose lacl ger ed, when each of the 2…

A: the lacI gene is a regulatory gene for lac operon It produces a protein that blocks RNA polymerase…

Q: Which of the following is false when the genes of the Lac operon are being tran- scribed in high…

A: Lac operon is a set of genes used by E.coli to metabolize lactose when glucose is not available for…

Q: The attenuation mechanism that helps block expression of the tryptophan operon requires all of the…

A: In transcriptional attenuation, the expression of genes are negatively regulated by controlling the…

Q: Which protein(s) would be bound to the DNA of the lac operon in the presence of both lactose and…

A: INTRODUCTION Lac operon This is group of genes having a single promoter. It contain protein that…

Q: 3. Draw an anabolic operon with three biosynthetic genes for compound Q and a catabolic operon with…

A:

Q: When lactose is first introduced into the medium, the lac operon is not induced and thus permease…

A: The lac operon in E.coli consists of 3 genes (lac Z, lac Y, lac A). The lac Z encodes beta…

Q: You can determine the regulation of an unknown operon based on descriptions like those in the table…

A: Introduction  The expression of genes is highly regulated in both prokaryotes as well as in…

Q: A mutation that inactivates transcription and translation from the regulatory gene of an inducible…

A: An operon is a functional unit of genomic DNA that comprises a collection of genes that are all…

Q: cAMP binds to cAMP Receptor Protein (CRP), allowing CRP to bind to the promoter of the lac operon

A: Almost all bacteria such as E. coli contains lac operon which operates in utilization of lactose as…

Q: A lac operon containing one mutation was cloned into a plasmid, which was introduced by…

A: (a) The plasma i gene forms such a type of repressor that will not be able to bind the inducer.

Q: 1) The UR operon which is composed of sequences A, B, C, and D (which may or may not be genes of…

A: An operon may be considered as a group of certain genes which function together so as to regulate…

Q: Strain LOLZ has a mutation in the lacI gene that results in a lacI protein that cannot bind…

A: Lac operon is the segment of DNA which is responsible for breaking down lactose into glucose and…

Q: Compare the types of bacterial genes associated with inducible operons, those associated with…

A: Gene regulation is a collection of mechanisms that the cell employs to reduce or increase the…

Q: Imagine that a new strain of E. coli is discovered, and it contains the following novel genetic…

A: The lac operon is an operon, which is a collection of genes with a single promoter. The genes in the…

Q: Examine the following theoretical operon (gen) in E. coli which contains structural genes (genC,…

A: Question 1:The genB gene's translation would be prematurely stopped by a nonsense mutation in the…

Q: ou have isolated different mutants (reg1 and reg2) causing constitutive expression of the emu operon…

A: The operon's regulation can take place positively or negatively. If the negative operator is bound…

Q: The lac operon in E. coli bacteria codes for proteins that break down (digest) the disaccharide…

A: The lac operon is expressed at its highest levels when there is lactose but no glucose (Option C is…

Q: 2. (A) Describe the role that PI plays in transcriptional regulation of the ginALG operon and in the…

A: A.glnALG operon along with the glnD and glnF and their gene products plays an extremely important…

Q: Suppose you have six strains of E. coli. One is wildtype, and each of the other five has a single…

A: Escherichia coli (E.coli) has the following five mutant strains. Lac Z-, lacy-, lacl-, Oc, and…

Q: Bacteria with a lac phenotype are unable to use lactose because of some defect in expressing the…

A: Answers - 1) False 2) True 3) True  There are 3 structural genes in lac operon - lac Z, lac Y and…

Q: The symbols A, B, C, D, E represent all the components of the lac operon, excluding LacA. The…

A: The lac operon regulates gene expression in lactose metabolism in E. coli. It is composed of a…

Q: Which of the following environmental conditions would be MOST likely to result in transcription of…

A: The transcription of the lac Oberon in an E.colicell is high levels of lactose and high levels of…

Q: Read the following statements about operons, and mark the FALSE one. O a. Prokaryotes use operons…

A: Operons are a group of genes that are regulated together in prokaryotic cells. They consist of…

Q: What is the effect of a mutation in the allosteric site of the Lac Repressor protein?

A: Lac operon (often called lactose operon) is an important operon sequence required for the metabolism…

Q: Suppose that E. coli sustains a mutation in its gene for the lac operon depressor making the…

A: A lac operon is a group of genes with a single promoter ( it is transcribed as a single mRNA). The…

Q: Which of the following statements about operons are true? (Check all that apply) Operons are common…

A: Ans: An operon is a cluster of the genes found in bacteria and viruses, these genes transcribed to…

Q: Strain P77 has a mutation in the lacO that prevents it from being bound by the lacI protein.…

A: The operon model is applicable in bacterial gene regulation and also applicable in some lower…

Gene Interactions

When the expression of a single trait is influenced by two or more different non-allelic genes, it is termed as genetic interaction. According to Mendel's law of inheritance, each gene functions in its own way and does not depend on the function of another gene, i.e., a single gene controls each of seven characteristics considered, but the complex contribution of many different genes determine many traits of an organism.

Gene Expression

Gene expression is a process by which the instructions present in deoxyribonucleic acid (DNA) are converted into useful molecules such as proteins, and functional messenger ribonucleic (mRNA) molecules in the case of non-protein-coding genes.