Identify the applications for gel filtration chromatography. separation of components in a mixture by hydrophobicity estimation of molecular weights separation of components in a mixture by size purification of a specific protein from a mixture

Q: Dilution Problem. A culture of Staphylococcus is diluted as follows: (1) 70mL are added to 330mL of…

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Q: The graph below is a standard curve generated by plotting the distance travelled by the size…

A: The standard curve generated for an analysis involving separation of proteins along the SD-PAGE gel…

Q: What is ion-exchange chromatography based on? moving a molecule with a net charge in an electric…

A: Ion exchange means the exchange of ions with protein molecules. In chromatography, there is a…

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Q: In SDS page, the ________ proteins will move through the gel fastest and first, and in size…

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Q: You have a protein that is easily extracted from a cell membrane preparation, without any associated…

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Q: Part I. For each of the following four questions: calculate and describe how the requested solution…

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Q: ELISA Testing:  Give me a step by step summary of the order things bind to each other in a positive…

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Q: . For each of the following separation methods covered in class, describe the basics of how that…

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Q: Standard curves You are setting up a standard curve for lysozyme. You have a stock vial containing 2…

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Q: TONICITY The salinity (concentratin of NaCl) if human blood is 0.9%. Imagine an evil nurse injected…

A: Human blood has 0.9% salinity which is an example of isotonic solution.

Q: K.What would happen if you reversed the electrodes on the Gel box? What would you get look like…

A: Introduction Electrophoresis  distinguish the DNA fragments of different lengths. Electrodes is…

Q: agent). The type of binding that is disrupted by A) Peptide bonds B) Trans double bonds C) Cis…

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Q: 1. How many mL of sterile water must be added to make the preparation isotonic? 2. How many mL of…

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Q: Growth Problem. A culture with approximately 1x104 cells/mL were incubated. After 7 hours, the…

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Q: In which solvents or solutions will a steroid be soluble? Check all that apply. 9% aqueous LiBr…

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Q: Liquid supported membrane of DGA used for rare earth elements separation. Tell me the…

A: liquid-supported membrane of di-(2-ethylhexyl) phosphoric acid." It is a type of membrane used for…

Q: Growth Problem. A culture with approximately 1x104 cells/mL were incubated. After 7 hours, the…

A: Generation time in minutes: Time in hr *60: 7*60= 420 min. Time for 1 Generation 70 min No of…