2c. 2d. We know that most pigments are found in the chloroplasts of eukaryotic cells. Specifically WHERE is chlorophyll located in the chloroplast? Some PROKARYOTES are photosynthetic. Predict where the pigments might be located in these cells.

Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Activity #1. Separation and Identification of Pigments by Paper Chromatography
Given the following information about the molecular structure of photopigments,
number the pigments in the order you would expect them to separate on the
chromatograph. #1 should be the one that travels the farthest with the solvent
(thus, will be the highest band), #4 should be the one that travels the least (i.e.,
lowest band).
1a.
Chlorophyll a:
Chlorophyll b:
Carotene:
Xanthophyll:
1b. List the safety precautions that apply to this activity.
2a.
Activity #2. Determining Absorption Spectrum for Leaf Pigments
Use your textbook to help you answer these questions. Remember that a
pigment appears a certain color because it REFLECTS (i.e., does not absorb)
that wavelength.
What wavelength range would be REFLECTED by each of these pigments?
2b.
has 5 polar groups on the molecule
has 6 polar groups on the molecule
have no polar groups on the molecule
has 2 polar groups on the molecule
Chlorophyll a:
Chlorophyll b:
Carotene:
Xanthophyll:
● Chlorophyll a:
Chlorophyll b:
Carotene:
At approximately what two wavelengths would you expect the greatest
absorbance values for:
nm
nm
nm
nm (HINT: Xanthophyll is yellow)
10
nm and
nm and
nm and
nm
nm
nm
Transcribed Image Text:Activity #1. Separation and Identification of Pigments by Paper Chromatography Given the following information about the molecular structure of photopigments, number the pigments in the order you would expect them to separate on the chromatograph. #1 should be the one that travels the farthest with the solvent (thus, will be the highest band), #4 should be the one that travels the least (i.e., lowest band). 1a. Chlorophyll a: Chlorophyll b: Carotene: Xanthophyll: 1b. List the safety precautions that apply to this activity. 2a. Activity #2. Determining Absorption Spectrum for Leaf Pigments Use your textbook to help you answer these questions. Remember that a pigment appears a certain color because it REFLECTS (i.e., does not absorb) that wavelength. What wavelength range would be REFLECTED by each of these pigments? 2b. has 5 polar groups on the molecule has 6 polar groups on the molecule have no polar groups on the molecule has 2 polar groups on the molecule Chlorophyll a: Chlorophyll b: Carotene: Xanthophyll: ● Chlorophyll a: Chlorophyll b: Carotene: At approximately what two wavelengths would you expect the greatest absorbance values for: nm nm nm nm (HINT: Xanthophyll is yellow) 10 nm and nm and nm and nm nm nm
2c.
2d.
We know that most pigments are found in the chloroplasts of eukaryotic cells.
Specifically WHERE is chlorophyll located in the chloroplast?
Some PROKARYOTES are photosynthetic. Predict where the pigments might
be located in these cells.
Activity #3. Determining optimal wavelength of light used during spinach leaf
photosynthesis
Note: You'll need to read through this lab exercise and your textbook to answer these
questions.
Answer choices for questions #1-4
(Each answer can be used more than
ADP
ATP
CO₂
Glucose
H₂O
Light
NADP+
NADPH
02
3. Which of the above are directly required by the light reactions? Hint: See the
photosynthesis chapter in your textbook.
4. Which of the above are directly produced by the light reactions?
5. Which of the above are directly required by the Calvin cycle?
6. Which of the above are directly produced by the Calvin cycle?
11
7. (a) In our experiment, what will cause the leaf discs to float?
(b) The greater the number of floating discs, the (greater or lesser) the rate of
photosynthesis.
Transcribed Image Text:2c. 2d. We know that most pigments are found in the chloroplasts of eukaryotic cells. Specifically WHERE is chlorophyll located in the chloroplast? Some PROKARYOTES are photosynthetic. Predict where the pigments might be located in these cells. Activity #3. Determining optimal wavelength of light used during spinach leaf photosynthesis Note: You'll need to read through this lab exercise and your textbook to answer these questions. Answer choices for questions #1-4 (Each answer can be used more than ADP ATP CO₂ Glucose H₂O Light NADP+ NADPH 02 3. Which of the above are directly required by the light reactions? Hint: See the photosynthesis chapter in your textbook. 4. Which of the above are directly produced by the light reactions? 5. Which of the above are directly required by the Calvin cycle? 6. Which of the above are directly produced by the Calvin cycle? 11 7. (a) In our experiment, what will cause the leaf discs to float? (b) The greater the number of floating discs, the (greater or lesser) the rate of photosynthesis.
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