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I intend to purify the enzymes from the crude enzyme extract. The extract contains at least five enzymes with various molecular weights and net charges at pH 7: Molecular Weight (Da) Net charges at pH 7 Proteins/enzymes 48,500 5,000 250,000 150,000 A В ++ C D 60,500 aArrange the elution profile sequence of these enzymes in a Gel Filtration 'Chromatography from the first eluted to the last. Justify your answer. Arrange the elution profile sequence of these proteins in an Anion Exchange Chromatography from the first eluted to the last. Justify your E +

I intend to purify the enzymes from the crude enzyme extract. The extract contains at least five enzymes with various molecular weights and net charges at pH 7: Molecular Weight (Da) Net charges at pH 7 Proteins/enzymes 48,500 5,000 250,000 150,000 A В ++ C D 60,500 aArrange the elution profile sequence of these enzymes in a Gel Filtration 'Chromatography from the first eluted to the last. Justify your answer. Arrange the elution profile sequence of these proteins in an Anion Exchange Chromatography from the first eluted to the last. Justify your E +

Biochemistry
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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Idris has successfully extracted enzymatic proteins from the fish viscera (intestines and stomach). After homogenization and centrifugation, he managed to pool the crude enzyme extract. He is characterizing the enzymes. Please help Idris by answering the following
questions:

I intend to purify the enzymes from the crude enzyme extract. The extract contains at least five enzymes with various molecular weights and net charges at pH 7: Proteins/enzymes Molecular Weight Net charges at pH 7 (Da) 48,500 5,000 250,000 150,000 60,500 А В ++ C D E + Arrange the elution profile sequence of these enzymes in a Gel Filtration 'Chromatography from the first eluted to the last. Justify your answer. Arrange the elution profile sequence of these proteins in an Anion Exchange Chromatography from the first eluted to the last. Justify your answer. C) I am going to analyse my proteins qualitatively by running a Sodium Dodecyl Sulphate-Polyacrylamide Gel Electrophoresis (SDS-PAGE). V What is the principle behind this technique? V Arrange the protein bands from top to bottom on the SDS-PAGE gel.
Transcribed Image Text:I intend to purify the enzymes from the crude enzyme extract. The extract contains at least five enzymes with various molecular weights and net charges at pH 7: Proteins/enzymes Molecular Weight Net charges at pH 7 (Da) 48,500 5,000 250,000 150,000 60,500 А В ++ C D E + Arrange the elution profile sequence of these enzymes in a Gel Filtration 'Chromatography from the first eluted to the last. Justify your answer. Arrange the elution profile sequence of these proteins in an Anion Exchange Chromatography from the first eluted to the last. Justify your answer. C) I am going to analyse my proteins qualitatively by running a Sodium Dodecyl Sulphate-Polyacrylamide Gel Electrophoresis (SDS-PAGE). V What is the principle behind this technique? V Arrange the protein bands from top to bottom on the SDS-PAGE gel.
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