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The following data were obtained during batch growth of the Saccharomyces Cerevisiae at its optimal temperature on a fully define synthetic medium with glucose as the growth limiting nutrient. The organisms were observed to die exponentially. a) Use the data to determine if lag phase is existed? Explain your answer. b) What is the maximum specific growth rate (Hmax) and culture doubling time (ta) of the organism? c) Estimate the volumetric rate of substrate consumption (r,) at hour 3 given that the value of maintenance coefficient (m,) is 0.036 g glucose/g biomass.h and monod constant, Ks is 2 g/l.

The following data were obtained during batch growth of the Saccharomyces Cerevisiae at its optimal temperature on a fully define synthetic medium with glucose as the growth limiting nutrient. The organisms were observed to die exponentially. a) Use the data to determine if lag phase is existed? Explain your answer. b) What is the maximum specific growth rate (Hmax) and culture doubling time (ta) of the organism? c) Estimate the volumetric rate of substrate consumption (r,) at hour 3 given that the value of maintenance coefficient (m,) is 0.036 g glucose/g biomass.h and monod constant, Ks is 2 g/l.

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
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The following data were obtained during batch growth of the Saccharomyces Cerevisiae at its optimal temperature on a fully define synthetic medium with glucose as the growth limiting nutrient. The organisms were observed to die exponentially. a) Use the data to determine if lag phase is existed? Explain your answer. b) What is the maximum specific growth rate (µmax) and culture doubling time (ta) of the organism? c) Estimate the volumetric rate of substrate consumption (r;) at hour 3 given that the value of maintenance coefficient (m.) is 0.036 g glucose/g biomass.h and monod constant, Ks is 2 g/l. BTEN 2314 Biochemical Engineering Fundamentals Glucose conc. (g/l) Time (hour) Biomass conc. (g/l) 0.1 20 1 0.37 19.3 1.5 0.70 18.6 2 1.32 17.0 2.5 2.52 14.1 3 4.89 9.2 3.5 8.99 4.1 4.0 8.59 1.0 5 7.85 6 7.35 8 6.20 10 5.15 15 3.45 20 2.20
Transcribed Image Text:The following data were obtained during batch growth of the Saccharomyces Cerevisiae at its optimal temperature on a fully define synthetic medium with glucose as the growth limiting nutrient. The organisms were observed to die exponentially. a) Use the data to determine if lag phase is existed? Explain your answer. b) What is the maximum specific growth rate (µmax) and culture doubling time (ta) of the organism? c) Estimate the volumetric rate of substrate consumption (r;) at hour 3 given that the value of maintenance coefficient (m.) is 0.036 g glucose/g biomass.h and monod constant, Ks is 2 g/l. BTEN 2314 Biochemical Engineering Fundamentals Glucose conc. (g/l) Time (hour) Biomass conc. (g/l) 0.1 20 1 0.37 19.3 1.5 0.70 18.6 2 1.32 17.0 2.5 2.52 14.1 3 4.89 9.2 3.5 8.99 4.1 4.0 8.59 1.0 5 7.85 6 7.35 8 6.20 10 5.15 15 3.45 20 2.20
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