A genetically modified bacterium was cultivated to produce 1,3-propanediol (1,3-PDO), and the following yields were obtained: YX/S = 0.106 gX/gS and YP/S = 0.28 gP/gS. Knowing that the culture medium was formulated with glucose, ammonia and some mineral salts, and the cultivation was carried out under aerobic conditions, answer: a) What is the oxygen demand in this process? b) What is the maximum theoretical yield of 1,3-PDO in this process? c) Projecting the scale-up, what cell concentration must be reached to obtain 10 kg of 1,3-PDO in a batch reactor with 1 m3 of culture?
A genetically modified bacterium was cultivated to produce 1,3-propanediol (1,3-PDO), and the following yields were obtained: YX/S = 0.106 gX/gS and YP/S = 0.28 gP/gS. Knowing that the culture medium was formulated with glucose, ammonia and some mineral salts, and the cultivation was carried out under aerobic conditions, answer: a) What is the oxygen demand in this process? b) What is the maximum theoretical yield of 1,3-PDO in this process? c) Projecting the scale-up, what cell concentration must be reached to obtain 10 kg of 1,3-PDO in a batch reactor with 1 m3 of culture?
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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A genetically modified bacterium was cultivated to produce 1,3-propanediol (1,3-PDO), and the following yields were obtained: YX/S = 0.106 gX/gS and YP/S = 0.28 gP/gS. Knowing that the culture medium was formulated with glucose, ammonia and some mineral salts, and the cultivation was carried out under aerobic conditions, answer:
a) What is the oxygen demand in this process?
b) What is the maximum theoretical yield of 1,3-PDO in this process?
c) Projecting the scale-up, what cell concentration must be reached to obtain 10 kg of 1,3-PDO in a batch reactor with 1 m3 of culture?
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