Two flasks of E. coli are grown in batch culture in the same medium (2% glucose and amino acids; no nitrate) and at the same temperature (378C). Culture #1 is well aerated. Culture #2 is anoxic. After 16 hours the following observations are made: ■ Culture #1 has a high cell density; the cells appear to be in stationary phase, and the glucose level in the medium is reduced to 1.2%. ■ Culture #2 has a low cell density; the cells appear to be in logarithmic phase, although their doubling time is prolonged (over 1 hour). The glucose level is reduced to 0.2%. Why does culture #2 have so little glucose remaining relative to culture #1, even though culture #2 displayed slower growth and has less biomass?

Q: Calculate the CFU/ml of leuconostoc in the brine for the 4 different days. Show your calculation

A: CFU/ml stands for "colony forming units per milliliter." It is a unit of measurement used in…

Q: There are two cultures of yeast cells in the pictures, one has been incubated for 6 hours and one…

A: Let's say, the yeast cell culture which was incubated for 6  hours as  = sample A the yeast cell…

Q: Using one of the graphs obtained with the medium II, name the different phases of the bacterial…

A: Micro organisms are typically grown in a closed culture media. Nutrients are added according to the…

Q: Why do cells need to be kept in the freezing devices first then transferred to liquid nitrogen…

A: Rapid freezing of cells can cause ice crystals large crystals can be formed in which cells are…

Q: Which disinfectant is the most effective? and why? Which disinfectant(s) is (are) bactericidal? and…

A: Disinfectant A is most effective as  there is no growth of salmonella choleraesuis at dilution 1:2…

Q: This is the growth curve for Clostirdium (incubated under optimal growth conditions). Use it to…

A: There are 5 phases to a logistic (sigmoidal) growth curve.  They are stationary phase, lag phase,…

Q: ium for the Cultivation of Saccharomyces cerevisid Component Value Unit g/L g/L lucose 5 east…

A: Algae are a diverse group of aquatic organisms with specific characteristics with the  ability to…

Q: Describe how you would prepare a dilution series of a 1 x 107 CFU/mL culture to the 10-8 dilution…

A: Serial dilutions is a process of making step by step dilutions of a substance. It is formed by…

Q: In a fed-batch culture operating with intermittent addition of lactose solution, values ofthe…

A: Note: As per Bartleby Guidelines For Remaining Answers Please Repost The Question. Introduction:  A…

Q: E. coli has a doubling time of 0.345 hours at 37 0C. Starting with 1 milligram of cells, assuming a…

A: Microorganisms have complex interactions with their environment and with other microorganisms, and…

Q: Below is shown a growth curve for an E. coli culture. As indicated, the culture was incubated in the…

A: In part (b) of the E. coli growth curve scenario, we're analyzing the potential binding of CAP…

Q: Menaquinones is produced by Flavobacterium meningosepticum via submerged fermentation with 60%…

A: Menaquinone are commonly known as vitamin K2. The structure of compound indicates that the compound…

Q: ing is a recipe for a bacterial growth medium: 35 g Dextrose, 12 g Aga 1.2g Salt, 3 g Yeast cell…

A: Growth medium can be of many types. Growth media is a media which contains nutrients for chemical…

Q: Before starting the freeze-drying process, the weight of the test tube, Wi which is 15.33g and test…

A: Given, Weight of the test tube is 15.33g Test tube weight with microbial cell pellets is 34.94g…

Q: CFU

A: CFU:It stands stands for Colony-Forming Unit. It is a measure used to estimate the number of viable…

Q: The cell concentration measured in samples taken at certain intervals from a microorganism growth…

A: From the given table,   Cf, final cell concentration =0.376  Ci, initial cell concentration=0.01…

Q: You have 0.05 ml of kombucha that contains microbes at a concentration of 8.2 x 10^6 CFU/ml. You…

A: Creating proper dilutions and working out the colony-forming units or CFU/ ml is one integral part…

Q: What would you need to culture the following microorganisms? (Note the hints and/or choices after…

A: Thermophile - Organisms tolerant to high temperature  Mesophile - organisms tolerant to moderate…

Q: What is the amount (mL) of pre-culture (108 cell/mL) necessary to inoculate (to add) in a 2-liter…

A: Culture media is a solid or semisolid substance used to support the growth of microorganisms. It is…

Q: Which of the the following results of the extracellular enzyme tests is FALSE?    Question 3…

A: Answer: Extracellular Enzymes or Exoenzymes are the enzymes secreted by the organisms or cells and…

Q: Growth of an E. coli bacteria in batch culture is observed. The bacteria are fed with 0.5 g / L…

A: Answer: E.coli = These are the gram negative bacteria can be found in normal human gut flora.

Q: As bacteria grow, they produce acidic metabolic waste products that gradually slow down the rate of…

A: Bacteria are unicellular organisms that cannot be seen from the naked eyes. They are present in a…

Q: The students of a Microbiology class were tasked to transfer or subculture a pure culture of…

A:

Q: Solve the following problem: 34,000 bacteria/l have been measured 4 hours after inoculating a…

A: Given information Bacterial population after 4 hours of inoculation= 34,000 bacteria/l Bacterial…

Q: Growth of an E. coli bacteria in batch culture is observed. The bacteria are fed with 0.5 g / L…

A: Bacteria are microorganisms that grow very fast. They almost double every 20 to 30 minutes.…

Q: Answer the following questions briefly and concisely 1.How do bacteria in a chemostat and those in…

A: If the dilution rate in a chemostat is higher than the organisms maximum specific growth rate it…

Q: A milk sample was tested for the presence of typhimurium. Using a 10 microliter calibrated loop, a…

A: Serial dilution is the technique to find the concentration of bacteria in a sample when there are…

Q: What is the advantage of the plating method over an electronic cell counting method in counting…

A: Bacteria culturing is done in order to grow the bacterial strains that are able to show resistance…

Q: A culture consisting of 100 litres of nutrient medium containing 12 g l-1 of growth limiting…

A: Biomass is organic matter that has been recently living or that is capable of decomposing to release…

Q: Medium used for cellulase production contained (g/L): NaNO3, 3; MgSO4 7H2O, 0.5; K2HPO4, 1; KCl, 0.5…

A: Culture media is proper nutrient for the growth of the microorganisms, which help to grow a variety…

Q: Draw a semi-logarithmic diagram showing the killing kinetics of a vegetative bacterial culture,…

A: Because they can inactivate vegetative cells, bacterial spores, yeast, and moulds, thermal…

Q: An otic product was subjected to AET and tested with the challenge microorganisms. An initial…

A: AET or antimicrobial effectiveness testing is a method often carried out in food technology and…

Q: In growing E.coli, why is that (reasons) they do not grow after doubling time under 20 degrees…

A: E. coli is a mesophilic bacteria. It functions under certain ideal temperatures and pressure. The…

Q: If the volume of a Staphylococcus aureus cell is estimated at 0.5 μm3, how many cells could be…

A: Staphylococcus aureus is a gram-positive bacteria, the cell wall is made up of a dense peptidoglycan…

Q: Explain how to prepare 250 ml liquid and 500 ml solid form separately from LB (Luria Bertani) medium…

A: The LB agar medium is designed in such a way that it is nutrient rich which supports the growth of…

Q: Define what is a minimal medium and the nature of its components.

A: A culture medium can be defined as solid,liquid or semi solid medium which helps in support of the…

Q: what is the decimal reduction time?

A: D-value or the Decimal reduction rate is the required time to achieve 90% killing/ reduction of…

Microbial Growth

The development of microbial growth is influenced by various natural conditions (both physical and synthetic) like pH, temperature, saltiness, gas focus (O2, CO2, and H2), nutrients, water action, and assimilation. Knowing the distinctive physical and chemical factors that influence the development and endurance of microbial growth in the climate will assist with reinforcing endeavors outfitted towards their control. These lines lessen or diminish their harmful impacts on humankind, creatures, and the climate.