Hexokinase in red blood cells has a Km of approximately 50 µM. What concentration of blood glucose would yield a velocity equal to 90 % Vmax?
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- Use MS Excel to plot and determine the terms being asked: Please insert the excel file, thank you For each V (mmol/min), determine the value of [S] (mM) if Vmax = 5 mmol/min. Show your calculations. Using MS Excel, plot [S] (mM) versus V (mmol/min). Draw a line corresponding to the given Vmax parallel the x-axis and extend your plotted line to show its approach to Vmax. Provide your final plot [S] (mM) V (mmol/min) 10 1.2 1.7 2.1 2.2 2.5 Determine the values of Km and Vmax for the decarboxylation of β-keto acid given the following data. Please show and label your plot. Show your calculations and box your final [S] in mol/l V in mM/ min 2.500 0.588 1.000 0.500 0.714 0.417 0.526 0.370 0.250 0.256What is the Keq for the conversion of Glucose 6-Phosphate to Glucose 1-Phosphate if the phosphate transfer potential for Glucose 1-Phosphate and Glucose 6-Phosphate are -20.9 kJ/mol and -13.8 kJ/mol respectively? Select an answer and submit. For keyboard navigation, use the up/down arrow keys to select an answer. a 0.27 b 0.45 c 10.5 d 0.019 e 0.057 f 24.2 g 7.10 h 0.24 i 2477A biochemist is trying to determine the type of proteases they have isolated from walrus blubber. The three proteases and their relative activities in the presence of the indicated non-specific irreversible inhibitors are shown in the table below: Protease a Protease B Protease y Given this data, please answer the following question: The catalytic site of Protease ß contains an important: C R H + lodoacetate Normal Activity Normal Activity No Activity U + Tetranitromethane Normal Activity No Activity Normal Activity
- You plot a reaction progress curve and find the equation of the line to be y = -0.0961x + 0.7142. Calculate the U, U/μL and U/mL of lysozyme activity in this fraction. The lysozyme activity is done identical to what is indicated in the lab manual.Neutral sphingomyelinase 2 converts sphingomyelin into ceramide and phosphorcholine. What kind of enzyme is it? Assume Vmax is 35 µM min-1. When you provide 3.0 x 10-5 M of sphingomyelin, you observe an initial velocity of 6.0 µM min-1. Calculate the KM.The standard curve provided below will allow you to answer the remaining questions. Generic Standard Curve 0.7 y = 0.9425x- 0.0034 0.6 0.5 0.4 0.3 02 0.1 0.1 0.2 0.3 0.4 0.5 0.6 0.7 Concentration (mg/ml) - Please describe two ways in which you could use this standard curve to determine the concentration of a pigment in an unknown sample.| #1: #2: -What is your rough estimation of the concentration if you determine the absorbance of a sample is 0.150? Absorbance
- What is the x-intercept for the above reaction on a Lineweaver-Burk plot?A. -0.5 per mMB. -1.0 per mMC. -2.0 per mMD. -5.0 per mME. -10.0 per mMA glucose solution is supplied in a repeated fed batch culture. The following parameters have been measured at t=2.5 hours; the system was under a quasi-steady state at this time. V = 1.0L, F = 0.22L/hr, S_0 = 98g/L, mu_m = 0.31/hr, K_s = 0.14 g-glucose/L, YM_X/S = 0.48 g-cells/g-glucose, Xt_0 = 33g (1) Calculate the value of V0 and S. (2) Calculate the value of Xt for t = 2.5hrs.If a data from an enzyme experiment is plotted as a Lineweaver - Burk plot, and the Vmax is 0.02 sec/mol, and x-intercept is -2.5 mM-1, then what is the KM value?
- A flat (not spherical) tissue engineered skin (thickness 2.5 mm) is attached to the bottom of a dish and cultured with cell media atop it (which has an oxygen concentration of 0.15 mol/m3). The diffusivity of oxygen in the skin is 3.4 E-9 m2/s, and according to experiments that you’ve performed, you want to make sure that the concentration of oxygen in the device doesn’t fall below 0.06 mol/m3. (Below that concentration harms the cells.) The rate of oxygen consumption is -5.9 E-17 mol/[cell◦s]. What is the maximum cellularity (in cells/mL, three significant digits) that this tissue engineered skin can support?A flat (not spherical) tissue engineered skin (thickness 3.9 mm) is attached to the bottom of a dish and cultured with cell media atop it (which has an oxygen concentration of 0.15 mol/m3). The diffusivity of oxygen in the skin is 3.2 E-9 m?/s, and according to experiments that you've performed, you want to make sure that the concentration of oxygen in the device doesn't fall below 0.06 mol/m3. (Below that concentration harms the cells.) The rate of oxygen consumption is -5 E-17 mol/[cell•s]. What is the maximum cellularity (in cells/mL, three significant digits) that this tissue engineered skin can support?The enzyme phosphoserine phosphatase catalyses the reaction: phosphoserine + H2O → serine + Pi Kinetics measurements were performed using a range of Mg* concentrations and in the presence of different fixed concentrations of Ca-". The enzyme requires Mg²* for activity. The following data were recorded: [Ca²*] / mM 0.0 20.0 40.0 [Mg*]/ mM Initial velocity / µmol of product min 0.33 20.40 9.26 5.99 0.50 25.65 12.66 8.47 1.00 33.30 20.00 14.28 2.00 40.00 28.57 22.23 What type of inhibitor is Ca2* and what is its Kf? Suggest what role Mg** performs in the catalytic mechanism of phosphoserine phosphatase. Suggest how Ca2* inhibits the reaction.