Have a look at the plasmid map of PGEX-6P-1-InlA on page 11 of your prac guide. If you perform a restriction digestion on PGEX-6P-1-InlA with BamHI and Notl in order to cut out the In/A functional domain; what size will the two bands be if the digestion is run on an agarose gel? OA. 1380 and 4967 B. 1393 and 4954 C. 2339 and 4008 OD. 2339 and 4954
Have a look at the plasmid map of PGEX-6P-1-InlA on page 11 of your prac guide. If you perform a restriction digestion on PGEX-6P-1-InlA with BamHI and Notl in order to cut out the In/A functional domain; what size will the two bands be if the digestion is run on an agarose gel? OA. 1380 and 4967 B. 1393 and 4954 C. 2339 and 4008 OD. 2339 and 4954
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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![QUESTION 1
Have a look at the plasmid map of PGEX-6P-1-InIA on page 11 of your prac guide. If you perform a restriction digestion on
PGEX-6P-1-InlA with BamHI and Not/ in order to cut out the In/A functional domain; what size will the two bands be if the
digestion is run on an agarose gel?
A. 1380 and 4967
B. 1393 and 4954
C. 2339 and 4008
D. 2339 and 4954](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2F92756ff9-90e2-4c3c-90cf-dce0881ff676%2Fc49318d7-076f-456f-b43b-8475fd58a793%2Fhm9s72_processed.png&w=3840&q=75)
Transcribed Image Text:QUESTION 1
Have a look at the plasmid map of PGEX-6P-1-InIA on page 11 of your prac guide. If you perform a restriction digestion on
PGEX-6P-1-InlA with BamHI and Not/ in order to cut out the In/A functional domain; what size will the two bands be if the
digestion is run on an agarose gel?
A. 1380 and 4967
B. 1393 and 4954
C. 2339 and 4008
D. 2339 and 4954
![Apol (5322)
Apal (5258)
lacl
ApoI (4612)
tac promoter
pGEX-6P-1-INIA
6347 bp
PstI (3301)
Ampicillin
GST
C3 cleavage site
Apal (941)
Bam HI (946)
Pst I (1091)
Pst I (1412)
inlA funct dom
Pst I (1937)
NotI (2339)](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2F92756ff9-90e2-4c3c-90cf-dce0881ff676%2Fc49318d7-076f-456f-b43b-8475fd58a793%2Fy2f6vci_processed.png&w=3840&q=75)
Transcribed Image Text:Apol (5322)
Apal (5258)
lacl
ApoI (4612)
tac promoter
pGEX-6P-1-INIA
6347 bp
PstI (3301)
Ampicillin
GST
C3 cleavage site
Apal (941)
Bam HI (946)
Pst I (1091)
Pst I (1412)
inlA funct dom
Pst I (1937)
NotI (2339)
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