Given the following inforation, construuct te restrioction mapof a 2000 bP DNA FRAGment.   1. EcoRi Digestion produces 1100BP & 900 BP 2. PSTI Digestion produces 1500,300, and 200 BP 3. EcoRi+PstI togeter produces 800,700,300,200 BP

Q: Eco01091 2074 Pfol 46 BstAPI 170 Aatil 2017 Sspl 2501 Ndel 183 Ehel 235 Pdml 2204, Bogi 2215 Soal…

A: Restriction endonucleases also referred to as restriction enzymes are enzymes that cut DNA at…

Q: Calculate the concentration of plasmid DNA if OD260 value is 0.74 and OD280 value is 0.35 (taken…

A: Introduction A plasmid is a short extrachromosomal DNA molecule that can replicate independently of…

Q: digest suspect B's DNA using restriction enzyme 1. Restriction enzyme 1 cleaves the recognition site…

A: The restriction enzymes are endonucleases that digest DNA (deoxyribonucleic acid) molecules by…

Q: Single stranded DNA fragment of M13 phage DNA shown was mixed with the following  primers P1: AGTAG…

A: Polymerase chain reaction also abbreviated as PCR.A specific DNA fragment is quickly multiplied…

Q: Which step of the double Holliday junction pathway is directly responsible for ensuring that no…

A: For cells to survive, DNA double-strand breaks must be correctly repaired. The RecBCD mechanism of…

Q: A thermophillic bacterium was isolated from a hot spring at Perak to study its thermostable amylase…

A: DNA technology is the chemical manipulation of the genotype and the resulting phenotype of organisms…

Q: 2- Supposed that you have a DNA sequence sample with 10,000 bp. By using 2 different restriction…

A: The answer is 3.

Q: Do virtual restriction digests of 300ng pGLO plasmid using HinDIII, EcoRI, and XhoI separately, and…

A: DNA restriction enzymes cleaves the DNA at specific sites. These recognition sites have DNA…

Q: 83. A plasmid is cut with the restriction enzyme BamH1 giving fragments of 3000 and 1000 bp as…

A: In this plasmid, we got 2 fragments of length 1000 and 3000 bp after digestion with BamH1 that…

Q: Single and double digestion of plasmid pJC716 were performed using the restriction enzymes EcoRI and…

A: Plasmid is a double stranded circular dna molecule that is capable of replicating independently. It…

Q: During plasmid extraction, after the addition of acetate-containing neutralization buffer the large…

A: Plasmid extraction is a method for the isolation of DNA. In this method, purified plasmids are…

Q: which sample shows the plasmid is fully digested

A: 50 bp ladder sequence : 500 bp 450 bp 400 bp 350 bp 300 bp 250 bp 200 bp 150 bp 100 bp 50 bp

Q: The following plasmid is digested with EcoR1 and Ndel restriction endonucleases. Agarose…

A: Restriction endonucleases are enzymes which cleave the phosphodiester bonds within a DNA. These…

Q: 5 μL of plasmid DNA (pUC 19) was added to 50ul of CaCl2 competent E. coli that was then added 250 μL…

A: Introduction :- A plasmid is a small, circular, double-stranded DNA molecule that can replicate…

Q: Can you answer the last three q

A: Note: As per Bartleby Guidelines For Remaining Answers Please Repost The Question. Introduction:…

Q: B) Suppose, you had a linear DNA instead of circular DNA. The double stranded sequence of a…

A: Introduction :- Restriction enzymes, also known as restriction endonucleases, are enzymes that can…

Q: EcoRI Sacl Kpnl Aval ampR Xmal Smal lacZ BamHI Xbal Sall Accl HinclI Pstl Sphl HindIII Bam H1 Bam H1…

A: Answer :- Option (C) is correct. - 1. :-

Q: Hindl EcoRI ECORV BamHI 373 Salt Pstl. tet amp Plasmid 1000 DBR322 4361 be ori Ndel you were going…

A: Restriction enzymes or restriction endonucleases/exonucleases are enzymes capable of recognizing a…

Q: 3) Whole-mount in situ hybridization can be used to test for the function of non-coding DNA region.…

A: * Whole mount in situ hybridization is a tool that aids for temporal and spatial dissection of gene…

Q: During plasmid extraction, after the addition of acetate-conta in ing neutralization buffer the…

A: Plasmids are extrachromosomal DNA in the bacterial cells. They are double stranded circular DNA,…

Q: A short micrococcal nuclease digestion yielded DNA fractions of 700, 1400, and 2100 bp. Predict what…

A: Chromatin fibers have a 'beads on string' structure, and the string represents the DNA molecule…

Q: be used for a restriction fragment length polymorphism (RFLP) diagnostic assay to identify…

A: Polymerase chain reaction, or  as we say PCR, is a technique used to make many copies of a specific…

Q: Lane 1 = TakaraBio 200 bp DNA ladder Lane 2 = Haell only; (thick band) 1883 bp and 1871 bp, 799 bp…

A: Understanding the restriction digestion patterns of any plasmid is very important to know where…

Q: If a person os homozygous for the presence of an ALU repeat in the PV92 region you would expect the…

A: Homozygous refers to the presence of the same type of allele on a particular locus.

Q: When a segment of DNA containing either aVNTR or an RFLP is analyzed, the result is fragments of DNA…

A: DNA or deoxyribonucleic acid is a polymer of deoxyribonucleotides connected together via…

Q: All are propertles of nucleosomes EXCEPT: / Almal is eienskappe van nukleosome, behalwe: A. all are…

A: Nucleosome They are fundamental repeating subunits of all eukaryotic chromatin (except when…

Q: Given the Following DNA template, TAC   CGC   TCC   GCC  GTC   GAC   AAT   ACC   ACT, write out the…

A: The DNA template is used for the synthesis of mRNA molecules. mRNA molecule is used by the…

Q: Assume the samples in this diagram of gel electrophoresis are DNA molecules. Why do the samples…

A: Gel electrophoresis is used to separate molecules of different sizes. It uses charged molecules that…

Q: How many nanograms of DNA should we expect to be contained within the slowest band from the…

A: pGLO plasmid: pGLO is a plasmid vector mostly used for the expression of genes.  The pGLO plasmid…

Q: How many DNA fragments will result when the double-stranded DNA molecule below is digested with…

A: Since you have asked multiple questions, we will solve the first question for you. If you want any…

Q: Compare and contrast the effect of using large pore size (low %agarose) vs. small pore size (high%…

A: Agarose gel electrophoresis is a commonly used technique for separating DNA fragments based on their…

Q: Briefly explain why the total size of the pMBBS plasmid in the Restriction Enzymes practical is…

A: Plasmid:Circular mostlySize of plasmid is between <5kb - >200kbMay or may not be present in…

Q: If a uidA amplicon generateed by PCR is 200bp and the DNA fragments resulting from the restriction…

A: INTRODUCTION Polymerase Chain reaction This is the method of amplification of of nucleic acids such…

Q: One of the frist steps in isolating plasmid DNA via mini-prep is to pellet the cells after O/N…

A: 1) The use of Resuspension buffer in the extraction or the isolation of the plasmid from the DNA is…

Q: The recognition sequence for the restriction enzyme Sau3AIis GATC; in the recognition sequence…

A: Given; The recognition sequence for the restriction enzyme Sau3AI is GATC. The recognition sequence…

Q: The DNA polymerase used in PCR is taken from Thermus aquaticus, a bacterium that thrives in the…

A: Polymerase chain reaction (PCR) is a method to produce a large number of copies by amplifying the…

Q: Which RE can be used to distinguish bacterial strain 1 from strain 2 based on a sample DNA fragment…

A: Restriction enzymes are a type of nuclease that cuts DNA at a specific sequence of nucleotides. They…

Q: answer that attaced question

A: Restriction endonucleases are enzymes that act as molecular scissors and cut the DNA molecule at a…

Q: distinguish between bacterial cells that obtained the plasmid and those that did not

A: Bacteria are single celled organisms with a unique internal structure.

Q: In the very first round of PCR using genomic DNA,the DNA primers prime synthesis that terminates…

A: It is a quick and versatile in vitro molecular biology technique for amplifying the defined target…

Q: Transcribe and translate the mutated sequence #2. Determine the consequence, if any, for each…

A: DNA or Deoxyribonucleic acid serves as the genetic material of biological entities. It is composed…

Q: To determine the reproducibility of mutation fre-quency measurements, you do the following…

A: A gene is the essential physical and functional unit of heredity. Genes are comprised of DNA. A…