g mutations in a bacterial gene thatcodes for an enzyme whose amino acid sequence isknown. In the wild-type protein, proline is the fifthamino acid from the amino terminal end. In one ofyour mutants with nonfunctional enzyme, you find aserine at position number 5. You subject this mutant tofurther mutagenesis and recover three different strains.Strain A has a proline at position number 5 and actsjust like a wild-type strain. Strain B has tryptophanat position number 5 and also acts like wild type.Strain C has no detectable enzyme function at anytemperature, and you can’t recover any protein thatresembles the enzyme. You mutagenize strain C andrecover a strain (C-1) that has enzyme function. Thesecond mutation in C-1 that is responsible for therecovery of enzyme function does not map at theenzyme locus.a. What is the nucleotide sequence in both strands ofthe wild-type gene at this location?
g mutations in a bacterial gene that
codes for an enzyme whose amino acid sequence is
known. In the wild-type protein, proline is the fifth
amino acid from the amino terminal end. In one of
your mutants with nonfunctional enzyme, you find a
serine at position number 5. You subject this mutant to
further mutagenesis and recover three different strains.
Strain A has a proline at position number 5 and acts
just like a wild-type strain. Strain B has tryptophan
at position number 5 and also acts like wild type.
Strain C has no detectable enzyme function at any
temperature, and you can’t recover any protein that
resembles the enzyme. You mutagenize strain C and
recover a strain (C-1) that has enzyme function. The
second mutation in C-1 that is responsible for the
recovery of enzyme function does not map at the
enzyme locus.
a. What is the
the wild-type gene at this location?
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