From the above sequence I have copied the first 500bp of thesequence into Word.ATGGTAAAGGCCGTCGTCGGATTCGGCGCTGCATGCGGAATCTCTGCTATCGTTGCTTGCCTTTGGGCTGCACTTGTCATCACAAATGACATCAATGACATGTATGATGATGTGATGGGAGAGCTCGGAGGATTCAGAGATATCTCTGATGACACTTGGGGAACCCTTCTCGACGTTCGTCACGGAGCCGGAGAGTCTGCTGAGCAATACGTTCGTGGAATCTTCGGACGTCACAAGCGTTCCAACAGCCAATGCTCTTGCGGACTTCCATCTCAAGGATGCCCAGCCGGAGCTCCAGGAAACCCAGGAGCCCCAGGAGAGCCAGGAGGCACTGGACCAGACGGAAAGAACGGACCAACTGGACTTCCAGGACTTAACATTCCAATTCCAAATGACTTCCCTAAGGAGTGCATCAAGTGCCCAGCTGGACCACCAGGACAAGATGGACTTCCAGGACAAGAAGGATTCCAAGGACTTCCAGGAGACGCTGGAAAGCGTGG1) You now need to design primers to amplify this 500bpregion of the DNA. Write out the primer sequences in thecorrect orientation.2) Decide on two restriction sites that you can use to clonethis into pL4440's MCS. Identify their sequence.Tip: The plasmid map is in Figure 3, details of restrictionfound atsitesequencescanbehttps://enzymefinder.neb.com/#!#nebheader3) Add the restriction site DNA sequences to the correctend of each primer.promoterT7 promoter ccaGCACTGATATCATAGATCTAGAAL4440Xbal Spel Age! Noo! Nhe!GGAATTOGATATICAApaNotagGTÖGCGGCCGCTCcecAATT T7 promoterList the stepsrequired to clone the PCR prduct into the pL4440plasmid.Exact recipes and details of PCR mixes + machineconditions etc are not required, just try and think aboutthe whole procedure from start to finish.

Name: From the above sequence I have copied the first 500bp of thesequence
Uploaded: 2024-03-20T06:42:41.000Z
Channel: Bartleby
Description: From the above sequence I have copied the first 500bp of thesequence into Word.ATGGTAAAGGCCGTCGTCGGATTCGGCGCTGCATGCGGAATCTCTGCTATCGTTGCTTGCCTTTGGGCTGCACTTGTCATCACAAATGACATCAATGACATGTATGATGATGTGATGGGAGAGCTCGGAGGATTCAGAGATATCTCTGATGACACTTGGGGAACCCTTCT