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Fragment Fragment size, kb ECORI BamHI Fragment size, kb Fragment digest digest A' 21.2 -16.8 B -12.1 F/A D' 7.4 -7.2 E 5.8- 6.8 + 6.5 -5.6+ 5.5 D, F А, С 5.6- 4.9 B' 3.5 Fragments with very similar sizes form but one band on a gel. (a) Experimental determination of fragmentation pattems, resulting from enzymatic digestion of the 48.5-kb linear DNA molecule from phage A. Restriction digests are subjected to agarose gel electrophoresis, and the fragments are visualized under ultraviolet light after staining the gel with ethidium bromide, a fluorescent dye. • FIGURE 21.14 Fragmentation of bacteriophage A DNA with restriction endonucleases EcoRI or BamHI. Mapping the restric- tion sites also requires data from partial digestion or with digestion of the DNA with EcoRI and BamHI together (not shown). BamHI sites A DNA BamHI segments A B E EcoRI sites A DNA EcoRI segments A' B' C' D' E' E' 10 20 30 40 48.5 DNA length, kb (b) Restriction maps of cleavage sites for each enzyme on the DNA molecule. By convention, the fragments are assigned letters as shown. In this experiment the 12.1-kb fragment in the BamHI digest resulted from linking the A and F (terminal) fragments by base pairing at their cohesive ends (Chapter 23).

Fragment Fragment size, kb ECORI BamHI Fragment size, kb Fragment digest digest A' 21.2 -16.8 B -12.1 F/A D' 7.4 -7.2 E 5.8- 6.8 + 6.5 -5.6+ 5.5 D, F А, С 5.6- 4.9 B' 3.5 Fragments with very similar sizes form but one band on a gel. (a) Experimental determination of fragmentation pattems, resulting from enzymatic digestion of the 48.5-kb linear DNA molecule from phage A. Restriction digests are subjected to agarose gel electrophoresis, and the fragments are visualized under ultraviolet light after staining the gel with ethidium bromide, a fluorescent dye. • FIGURE 21.14 Fragmentation of bacteriophage A DNA with restriction endonucleases EcoRI or BamHI. Mapping the restric- tion sites also requires data from partial digestion or with digestion of the DNA with EcoRI and BamHI together (not shown). BamHI sites A DNA BamHI segments A B E EcoRI sites A DNA EcoRI segments A' B' C' D' E' E' 10 20 30 40 48.5 DNA length, kb (b) Restriction maps of cleavage sites for each enzyme on the DNA molecule. By convention, the fragments are assigned letters as shown. In this experiment the 12.1-kb fragment in the BamHI digest resulted from linking the A and F (terminal) fragments by base pairing at their cohesive ends (Chapter 23).

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Consider the λ bacteriophage DNA molecule as shown in Figure 21.14. Total digestion with the two restriction endonucleases used does not allow unambiguous placement of restriction sites, as shown in the map. Describe restriction patterns—either from partial digestion or from digestion with both BamHI and EcoRI—that would help to establish the specific map of restriction sites shown in Figure 21.14(b).

Fragment Fragment size, kb ECORI BamHI Fragment size, kb Fragment digest digest A' 21.2 -16.8 B -12.1 F/A D' 7.4 -7.2 E 5.8- 6.8 + 6.5 -5.6+ 5.5 D, F А, С 5.6- 4.9 B' 3.5 Fragments with very similar sizes form but one band on a gel. (a) Experimental determination of fragmentation pattems, resulting from enzymatic digestion of the 48.5-kb linear DNA molecule from phage A. Restriction digests are subjected to agarose gel electrophoresis, and the fragments are visualized under ultraviolet light after staining the gel with ethidium bromide, a fluorescent dye. • FIGURE 21.14 Fragmentation of bacteriophage A DNA with restriction endonucleases EcoRI or BamHI. Mapping the restric- tion sites also requires data from partial digestion or with digestion of the DNA with EcoRI and BamHI together (not shown). BamHI sites A DNA BamHI segments A B E EcoRI sites A DNA EcoRI segments A' B' C' D' E' E' 10 20 30 40 48.5 DNA length, kb (b) Restriction maps of cleavage sites for each enzyme on the DNA molecule. By convention, the fragments are assigned letters as shown. In this experiment the 12.1-kb fragment in the BamHI digest resulted from linking the A and F (terminal) fragments by base pairing at their cohesive ends (Chapter 23).
Transcribed Image Text:Fragment Fragment size, kb ECORI BamHI Fragment size, kb Fragment digest digest A' 21.2 -16.8 B -12.1 F/A D' 7.4 -7.2 E 5.8- 6.8 + 6.5 -5.6+ 5.5 D, F А, С 5.6- 4.9 B' 3.5 Fragments with very similar sizes form but one band on a gel. (a) Experimental determination of fragmentation pattems, resulting from enzymatic digestion of the 48.5-kb linear DNA molecule from phage A. Restriction digests are subjected to agarose gel electrophoresis, and the fragments are visualized under ultraviolet light after staining the gel with ethidium bromide, a fluorescent dye. • FIGURE 21.14 Fragmentation of bacteriophage A DNA with restriction endonucleases EcoRI or BamHI. Mapping the restric- tion sites also requires data from partial digestion or with digestion of the DNA with EcoRI and BamHI together (not shown). BamHI sites A DNA BamHI segments A B E EcoRI sites A DNA EcoRI segments A' B' C' D' E' E' 10 20 30 40 48.5 DNA length, kb (b) Restriction maps of cleavage sites for each enzyme on the DNA molecule. By convention, the fragments are assigned letters as shown. In this experiment the 12.1-kb fragment in the BamHI digest resulted from linking the A and F (terminal) fragments by base pairing at their cohesive ends (Chapter 23).
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