Fluorescence intensity was taken of 2.0 mL of the 10 μg/mL quinine standard solution into five 25 mL volumetric flasks. Into one, place 1.0 mL of 0.05 M sodium bromide solution and dilute to the mark with 0.05 M sulfuric acid solution. Similarly, add 2.0, 4.0, 8.0, and 16.0 mL of the sodium bromide solution to the remaining flasks before diluting to volume with the sulfuric acid solution. Why did the fluorescence intensity decrease as the concentration of sodium bromide increased?
Fluorescence intensity was taken of 2.0 mL of the 10 μg/mL quinine standard solution into five 25 mL volumetric flasks. Into one, place 1.0 mL of 0.05 M sodium bromide solution and dilute to the mark with 0.05 M sulfuric acid solution. Similarly, add 2.0, 4.0, 8.0, and 16.0 mL of the sodium bromide solution to the remaining flasks before diluting to volume with the sulfuric acid solution. Why did the fluorescence intensity decrease as the concentration of sodium bromide increased?
Chemistry
10th Edition
ISBN:9781305957404
Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Chapter1: Chemical Foundations
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Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...
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Fluorescence intensity was taken of 2.0 mL of the 10 μg/mL quinine standard solution into five 25 mL volumetric flasks. Into one, place 1.0 mL of 0.05 M sodium bromide solution and dilute to the mark with 0.05 M sulfuric acid solution. Similarly, add 2.0, 4.0, 8.0, and 16.0 mL of the sodium bromide solution to the remaining flasks before diluting to volume with the sulfuric acid solution. Why did the fluorescence intensity decrease as the concentration of sodium bromide increased?
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