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A student weighed out 0.150 g of protein powder and dissolved it in 100 mL of water (Solution 1). The student then quantitatively transferred 1 ml of Solution 1 into a 25 ml volumetric flask and diluting to the calibration line with DI water (Solution 2). Using the Bradford method, a 1 ml sample of Solution 2 was determined to have an absorbance of 0.349. If the best fit linear line of the standard calibration curve created in Part A was

A student weighed out 0.150 g of protein powder and dissolved it in 100 mL of water (Solution 1). The student then quantitatively transferred 1 ml of Solution 1 into a 25 ml volumetric flask and diluting to the calibration line with DI water (Solution 2). Using the Bradford method, a 1 ml sample of Solution 2 was determined to have an absorbance of 0.349. If the best fit linear line of the standard calibration curve created in Part A was

Chemistry
Chemistry
10th Edition
ISBN:9781305957404
Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Chapter1: Chemical Foundations
Section: Chapter Questions
Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...
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Question 6 A student weighed out 0.150 g of protein powder and dissolved it in 100 mL of water (Solution 1). The student then quantitatively transferred 1 mL of Solution 1 into a 25 mL volumetric flask and diluting to the calibration line with DI water (Solution 2). Using the Bradford method, a 1 ml sample of Solution 2 was determined to have an absorbance of 0.349. If the best fit linear line of the standard calibration curve created in Part A was y = 0.00008144 + 0.00521 (, assuming 1 mL samples for the Bradford method), calculate mL the percent protein by mass in the original protein powder. Report your answer with two places after the decimal.
Transcribed Image Text:Question 6 A student weighed out 0.150 g of protein powder and dissolved it in 100 mL of water (Solution 1). The student then quantitatively transferred 1 mL of Solution 1 into a 25 mL volumetric flask and diluting to the calibration line with DI water (Solution 2). Using the Bradford method, a 1 ml sample of Solution 2 was determined to have an absorbance of 0.349. If the best fit linear line of the standard calibration curve created in Part A was y = 0.00008144 + 0.00521 (, assuming 1 mL samples for the Bradford method), calculate mL the percent protein by mass in the original protein powder. Report your answer with two places after the decimal.
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