DNA QUANTITATION If you have the extracted/purified DNA and will not be immediately loaded in the spectrophotomer, how can you maximize the storage life of DNA? What practices will maximize the quality of your DNA sample?

Q: Restriction digestion and Gel electrophoresis: A single strand of a double-stranded DNA sequence is…

A: Restriction digestion is a process in which DNA is cut into smaller pieces at specific sites with…

Q: DNA extraction, PCR, gel electrophoresis, and DNA sequencing of PCR products. Give a description of…

A: DNA extraction is the procedure of isolating the Deoxyribonucleic acid from biological samples. PCR…

Q: DNa Mapping using Restriction enzymes lab: We will be aliquoting and delivering 5 μl of enzyme to…

A: A restriction enzyme, also known as restriction endonuclease is an enzyme that cleaves DNA into…

Q: Quantitation of the evidence DNA extracts from the Mockingbird Lane case return the following…

A: Polymerase Chain Reaction is a technique which is used to make multiple copies of a specific segment…

Q: Lvris and Irelation of DNA from Ranana)

A: To study the genetic build up of an organism, it is important to know the traits it possesses. Based…

Q: A DNA fragment with 450 bp will be closer to the top (negative pole) of an electrophoresis gel than…

A: Friedrich Meischer discovered nucleic acids from the nuclei of pus cells and coined the term…

Q: What's the main difference between first-generation sequencing, second-generation sequencing and…

A: DNA sequencing is a research center procedure used to decide the specific arrangement of bases (A,…

Q: In DNA extracting. What is the purpose of clear shampoo in the DNA extraction buffer?

A: DNA is extracted from different sources to analyze and study the DNA sequence and diseases caused…

Q: DNA Fingerprinting A. What is an RFLP and where are they located within a gene? B. Why are RFLPs…

A: A. RFLP (Restriction Fragment Length Polymorphism):RFLP refers to the variations in the lengths of…

Q: When choosing a DNA extraction protocol, which of the following should be first taken into account?…

A: DNA extraction is a process of isolating DNA from biological samples, such as cells, tissues, or…

Q: DNA QUANTITATION Consult related references (textbooks or research articles) and list possible…

A: DNA quantitation refers to the process of measuring the amount of DNA present in a given sample.…

Q: a.What is the importance of centrifugation in step 3?  b.

A: Introduction: For the development of diagnostics and medications as well as for research into the…

Q: The gDNA sample that was isolated together with 12 other samples from other species were up for…

A: Please ask volume for the master mix as a separate question.  I can answer only 1 question,  as…

Q: L 1 2 3 Locus A Locus B L = Standard marker 1 = Suspect 1 2 = Suspect 2 3 = DNA from crime scene %3D…

A: The biotechnology is a branch of biology that deals with different techniques that help…

Q: DNA QUANTITATION Why is a reagent blank (containing no nucleic acids) is taken prior to measuring…

A: DNA quantitation refers to the process of measuring the amount of DNA present in a given sample.…

Q: Lab 12- Gel Electrophoresis If provided an image or sample gel, can you use the MMR/DNA Ladder to…

A: Answer :: Gel electrophoresis is a method used to separate macromolecules like DNA, RNA, and…

Q: Explain this picture. Topic central dogma under DNA replication

A: Semiconservative Replication System:It is a most accepted model. It produces two DNA helices in…

Q: The key catalytic amino acids of the lysozyme active site in the free enzyme are Glu 35 with a…

A: The catalytic mechanism of lysozyme involves both general acid and general base catalysis.

Q: True or false: During DNA isolation, ice cold 95% ethanol is used to digest the DNA into fragments…

A: During DNA isolation 95% ethanol is used for DNA precipitation not for DNA digestion and while we…

Q: Band, Band Size, and what bands corresponds?

A: The image shows gel electrophoresis containing DNA fragments of various sizes. Gel electrophoresis…

Q: 2. why contaminants must be avoided in DNA samples?

A: DNA contamination occurs when foreign DNA contaminates your intended DNA sample. DNA contamination…

Q: How do I fill in each column for each DNA fragment?

A: Using the distance travelled by DNA bands, the size of DNA fragments can be calculated. Here, the…

Q: Loading dye stains the DNA molecules and thus help to visualize it under UV light apparatus. O True…

A: DNA Loading dye is used to monitor the migration of DNA into the gel during electrophoresis.…

Q: Explain the Doyle and Doyle (1990) method of extraction. Identify which step removes specific…

A: Introduction: Since Friedrich Miescher's original DNA extraction in 1869, scientists have made…

Q: Gel Electrophoresis A. Why does DNA travel toward the positive electrode in the gel chamber? B. What…

A: Approach to finding a solution to the problem:1. Have a solid understanding of the fundamentals of…

Q: Experiment: DNA Extraction from Banana The procedures are attached below. Question: 1. Will the…

A: DNA is the genetic material that is presented inside the nucleus of every cell. Banana is the best…

Q: Answer choices : 0.3% gel OR 1.0% gel Explanation Choices : Agarose makes smaller pore sizes which…

A: Electrophoresis is an analytical technique that separates the biomolecule on the gel matrix based on…

Q: DNA QUANTITATION Can you use the spectrophotometer to quantify proteins? Consult related references…

A: Quantitative methods are used to determine the quantity of a substance in a given sample. A…

Q: As a scientist in Company X, your manager asks you to design a methodology to separate the three DNA…

A: DNA or deoxyribonucleic acid is the genetic material that contains information that is passed from…

Q: Gel electrophoresis is a laboratory technique that separates molecules by size using an electric…

A: The laboratory method used to separate the mixtures of DNA, RNA or proteins according to their…

Q: DNA Isolation A. What is “cell lysis” and why would you want to lyse cells when doing a DNA…

A: A. Cell lysis is the process of breaking open the cell membrane to release the cellular contents,…

Q: Which of the following gels is the preferred choice for separation of DNA fragments? Agarose O…

A: Deoxyribonucleic acid, or DNA, is a molecule that contains enable an organism to grow, survive, and…

Q: 1.724 1.708 IA

A: Equilibrium Density Centrifugation of DNA -- Radio Active Labelling --- Radioactive labeling of…

Q: DNA QUANTITATION Explain the rationale behind using the A260/A280 in the spectrophotometric…

A: Nucleic acid is any group of long, linear macromolecules that carry hereditary information directing…

Q: The current state-of-the-art in forensic DNA profiling involves the PCR-amplification and analysis…

A: PCR is a process in which millions of copies of a specific sequence of DNA can be made in a matter…

Q: step 4, isopropyl alcohol is used to precipitate out the DNA because it is not soluble. What does…

A: Introduction: Pure DNA is needed for DNA analysis. In cells, DNA can be found. However, cells also…

Q: Identify the component of the solution which is the extracted DNA

A: Everything living contains DNA. This DNA can be isolated for a variety of molecular biology…

Q: Extracting DNA From Strawberries What is the purpose of adding the extraction buffer (soap + salt)?…

A: Strawberries are octoploid that means they have 8 copies of each chromosome in the cell.…

Q: What are the benefits of fluorescence labeling versus radioactive labeling of DNA?

A: Recently, labelling techniques are so advanced in imaging biomolecules. Fluorescence labeling,…

Q: DNA Restriction, Electrophoresis 1. What are restriction enzymes?

A: The development in biotechnology paves the way for recombinant DNA technology, which contributed to…

Q: There are 4 protocols that are being optimized for the isolation of genomic DNA from the larvae of a…

A: Agarose gel electrophoresis: Separates DNA fragements based on size