There are 4 protocols that are being optimized for the isolation of genomic DNA from the larvae of a newly discovered butterfly species. The results of the isolation experiments were verified via agarose gel electrophoresis and are shown below: 1kb DNA Protocol Protocol Protocol Protocol ladder + A B D 25,000 10,000 8,000 6,000 5,000 4,000 3,000 2,500 2,000 1,500 1,000 750 500 250 Based on the gel profile shown above, which protocol do you think is the best for the butterfly larvae 9DNA isolation? Explain the basis of your chosen protocol. Why did you not choose the other 3 protocols? ||

Basic Clinical Laboratory Techniques 6E
6th Edition
ISBN:9781133893943
Author:ESTRIDGE
Publisher:ESTRIDGE
Chapter2: Basic Hematology
Section2.3: Microhematocrit
Problem 3RQ
icon
Related questions
Question
100%

helping tags: biochemistry, agarose gel electrophoresis, genomic DNA isolation, gel electrophoresis

Please explain your answers thoroughly. Thanks will upvote.

There are 4 protocols that are being optimized for the isolation of genomic DNA from
the larvae of a newly discovered butterfly species. The results of the isolation
experiments were verified via agarose gel electrophoresis and are shown below:
1kb DNA
Protocol
Protocol
Protocol
Protocol
ladder +
A
B
D
25,000
10,000
8,000
6,000
5,000
4,000
3,000
2,500
2,000
1,500
1,000
750
500
250
Based on the gel profile shown above, which protocol do you think is the best for the
butterfly larvae 9DNA isolation?
Explain the basis of your chosen protocol.
Why did you not choose the other 3 protocols?
||
Transcribed Image Text:There are 4 protocols that are being optimized for the isolation of genomic DNA from the larvae of a newly discovered butterfly species. The results of the isolation experiments were verified via agarose gel electrophoresis and are shown below: 1kb DNA Protocol Protocol Protocol Protocol ladder + A B D 25,000 10,000 8,000 6,000 5,000 4,000 3,000 2,500 2,000 1,500 1,000 750 500 250 Based on the gel profile shown above, which protocol do you think is the best for the butterfly larvae 9DNA isolation? Explain the basis of your chosen protocol. Why did you not choose the other 3 protocols? ||
Expert Solution
steps

Step by step

Solved in 4 steps

Blurred answer
Similar questions
  • SEE MORE QUESTIONS
Recommended textbooks for you
Basic Clinical Laboratory Techniques 6E
Basic Clinical Laboratory Techniques 6E
Biology
ISBN:
9781133893943
Author:
ESTRIDGE
Publisher:
Cengage
Case Studies In Health Information Management
Case Studies In Health Information Management
Biology
ISBN:
9781337676908
Author:
SCHNERING
Publisher:
Cengage
Human Heredity: Principles and Issues (MindTap Co…
Human Heredity: Principles and Issues (MindTap Co…
Biology
ISBN:
9781305251052
Author:
Michael Cummings
Publisher:
Cengage Learning
Biology 2e
Biology 2e
Biology
ISBN:
9781947172517
Author:
Matthew Douglas, Jung Choi, Mary Ann Clark
Publisher:
OpenStax
Concepts of Biology
Concepts of Biology
Biology
ISBN:
9781938168116
Author:
Samantha Fowler, Rebecca Roush, James Wise
Publisher:
OpenStax College
Biology: The Dynamic Science (MindTap Course List)
Biology: The Dynamic Science (MindTap Course List)
Biology
ISBN:
9781305389892
Author:
Peter J. Russell, Paul E. Hertz, Beverly McMillan
Publisher:
Cengage Learning