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Direct Test: Positive Result Fluorescent dye molecule -Fluorescently labeled antibody (known specificity) Add fluorescently labeled antibodies of known specificity; wash off those that do not bind. View using fluorescence microscope. Unknown antigen Slide surface Unknown antigen attached to slide. Fluorescently labeled antibodies bind to antigen. View using fluorescence microscope. (a) Indirect Test: Positive Result -Fluorescently labeled Fluorescent Antibody- in serum (IgG) dye molecule anti-human Add patient's serum; wash off antibodies that do Add fluorescently labeled anti-human IgG; wash off any that do not bind. Known antigen IgG not bind. Slide surface Known antigen attached to slide. Patient's antibodies bind to antigen. Fluorescently labeled secondary antibodies bind to IgG. (b) FIGURE 18.6 Fluorescent Antibody (FA) Test (a) Positive direct FA test. (b) Positive indirect FA test.

Direct Test: Positive Result Fluorescent dye molecule -Fluorescently labeled antibody (known specificity) Add fluorescently labeled antibodies of known specificity; wash off those that do not bind. View using fluorescence microscope. Unknown antigen Slide surface Unknown antigen attached to slide. Fluorescently labeled antibodies bind to antigen. View using fluorescence microscope. (a) Indirect Test: Positive Result -Fluorescently labeled Fluorescent Antibody- in serum (IgG) dye molecule anti-human Add patient's serum; wash off antibodies that do Add fluorescently labeled anti-human IgG; wash off any that do not bind. Known antigen IgG not bind. Slide surface Known antigen attached to slide. Patient's antibodies bind to antigen. Fluorescently labeled secondary antibodies bind to IgG. (b) FIGURE 18.6 Fluorescent Antibody (FA) Test (a) Positive direct FA test. (b) Positive indirect FA test.

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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In this diagram, why is anti-human IgG used in the indirect test but not in the direct test?

Direct Test: Positive Result Fluorescent dye molecule -Fluorescently labeled antibody (known specificity) Add fluorescently labeled antibodies of known specificity; wash off those that do not bind. View using fluorescence microscope. Unknown antigen Slide surface Unknown antigen attached to slide. Fluorescently labeled antibodies bind to antigen. View using fluorescence microscope. (a) Indirect Test: Positive Result -Fluorescently labeled Fluorescent Antibody- in serum (IgG) dye molecule anti-human Add patient's serum; wash off antibodies that do Add fluorescently labeled anti-human IgG; wash off any that do not bind. Known antigen IgG not bind. Slide surface Known antigen attached to slide. Patient's antibodies bind to antigen. Fluorescently labeled secondary antibodies bind to IgG. (b) FIGURE 18.6 Fluorescent Antibody (FA) Test (a) Positive direct FA test. (b) Positive indirect FA test.
Transcribed Image Text:Direct Test: Positive Result Fluorescent dye molecule -Fluorescently labeled antibody (known specificity) Add fluorescently labeled antibodies of known specificity; wash off those that do not bind. View using fluorescence microscope. Unknown antigen Slide surface Unknown antigen attached to slide. Fluorescently labeled antibodies bind to antigen. View using fluorescence microscope. (a) Indirect Test: Positive Result -Fluorescently labeled Fluorescent Antibody- in serum (IgG) dye molecule anti-human Add patient's serum; wash off antibodies that do Add fluorescently labeled anti-human IgG; wash off any that do not bind. Known antigen IgG not bind. Slide surface Known antigen attached to slide. Patient's antibodies bind to antigen. Fluorescently labeled secondary antibodies bind to IgG. (b) FIGURE 18.6 Fluorescent Antibody (FA) Test (a) Positive direct FA test. (b) Positive indirect FA test.
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