Deletions in bacterial chromosomes give the following data:Region of deletionAlGene A activity+++A2АЗA4AS+++(i)Where is the gene located? Explain your answer.Name the scientific term that describes the appearance of a recessive phenotype dueto deletion of dominant gene.(ii)(iii) The phenotypic consequences of deletion depend on two factors. What are they?Give one example of human genetic disorder caused by chromosomal deletion byindicating the region of deletion in chromosome.(iv)

The gene is located in A3 region. Because deletion of the A3 region leads to the least activity of…

Answered: Deletions in bacterial chromosomes give…

Human Anatomy & Physiology (11th Edition)

11th Edition

ISBN:9780134580999

Author:Elaine N. Marieb, Katja N. Hoehn

Publisher:Elaine N. Marieb, Katja N. Hoehn

Chapter1: The Human Body: An Orientation

Section: Chapter Questions

Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...

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12) Draw a yeast knockout cassette. Label all required sequence features. a) Draw the target chromosome if recombination only occurs at one region of homology. b) Draw the target chromosome if recombination occurs using both regions of homology.
EC4. In a test-cross of trihybrid drosophila the following phenotypes and number of offspring were found. Without doing any calculations, answer the following questions: +++ 669 abc 653 ++c 121 ab+ 139 +b+ 2280 a+c 2215 +bc 3 a++ 2 a) What were the genotypes of the parental strains? (fill in) b). What is the gene order?
For a haploid fungus, the starting point in the biosynthesis of the amino acid arginine is Compound X, which is always present in and absorbed from the environment. The arginine biosynthetic pathway is: Enzyme A Enzyme B Enzyme Ç Compound X It is know that genes encoding enzymes A and C are on two different chromosomes. Compound Y Compound Z- Arginine A mutant strain of genotype a (lacking only enzyme A) is crossed to a mutant strain of genotype c (lacking only enzyme C) to generate a diploid strain. Sporulation (i.e. meiosis) is subsequently induced in the resulting diploid strain. What proportion of the spores (haploids formed by sporulation) is expected to grow on medium without arginine but supplemented with Compound Y? O 100% 50% 0% 25%
. A diploid strain of yeast was made by mating a haploidstrain with a genotype w−, x−, y−, and z− with a haploidstrain of opposite mating type that is wild type for thesefour genes. The diploid strain was phenotypically wildtype. Four different X-ray-induced diploid mutantswith the following phenotypes were produced fromthis diploid yeast strain. Assume a single new mutation is present in each strain.Strain 1 w− x+ y− z+Strain 2 w+ x− y− z−Strain 3 w− x+ y− z−Strain 4 w− x+ y+ z+When these mutant diploid strains of yeast go throughmeiosis, each ascus is found to contain only two viablehaploid spores.a. What kind of mutations were induced by X-rays tomake the listed diploid strains?b. Why did two spores in each ascus die?c. Are any of the genes w, x, y, or z located on thesame chromosome?d. Give the order of the genes that are found on thesame chromosome
Explain two ways that a chromosomal rearrangement can cause a position effect.
Which of the following genetic mechanism isn't used by bacteria in the transference of genetic resistance to antibiotics? Which of the following genetic mechanisms is not used by bacteria in the transfer of antibiotic resistance? Select one: O transduction O conjugation O through transposons O transformation O crossing over between homologous chromosomes Which of the following is not consider one of the main factors in the emergence of antibiotic resistance? O a. an increase in immuocompromised patients f O b. increase O c. failures on the staff O d. an increase in antibiotic resistance pathogens
What percentage of the DNA sites in yeast are accessible, assuming that the fraction of sites observed for GAL4 is typical? To how many base pairs of the 12-Mb yeast genome does this percentage correspond?
The completely synthetic yeast chromosome Syn IIIcontains a loxP site in the 3′ UTR of every gene thatis potentially nonessential to yeast survival. As youwill recall from Chapter 6, loxP sites are targets ofsite-specific recombination. The researchers who constructed Syn III included these loxP sites as a way to“scramble” the chromosome, meaning that parts ofthe chromosome could easily be deleted or rearranged.The goal of these investigations is to drive the evolution of Syn III so as to define a minimal genome thatcan support the life of this organism. Outline the experiment the researchers would do to scramble Syn IIIin order to define a minimal genome.
For questions 22 through 30, please refer to the pictures below: Normal B B B C D D D D' E E 22 23 24 B D G. Y D M H. B C E 25 26 27 28 B D Break through centromere B B A D 29 30 Identify the type of chromosomal aberration in each number above. Please refer to the choices below. Reciprocal translocation Robertsonian translocation Isochromosome Terminal deletion Interstitial deletion Duplication Pericentric inversion Ring chromosome Marker chromosome Paracentric inversion
You have a strain of Neurospora that is unable to synthesize histidine and thus requires H in the media in order to grow. You have isolated one revertant colony. Predict the expected proportion of the progeny that would be h+ if you cross the colony with the original mutant colony and the reversion occurred by each of the following mechanisms: Precise change of the mutated base back to its original base. A suppressor gene is mutated on a different chromosome A suppressor gene is mutated on the same chromosome but 10mu distant from the mutated gene. The mutant colony is crossed to a wild-type Neurospora colony and the following data are collected. 95% of all asci scored are h+ but 5% are h-. Which mechanism in part a is consistent with these data? Explain why and what has happened on a molecular level.
A yeast strain with a mutant spo11- allele has been isolated. The mutant allele is nonfunctional; it makes no spo11 protein. What do you suppose is the phenotype of this mutant strain?
Nine rII− mutants of bacteriophage T4 were used inpairwise infections of E. coli K(λ) hosts. Six of themutations in these phages are point mutations; theother three are deletions. The ability of the doubly infected cells to produce progeny phages in large numbers is scored in the following chart.1 2 3 4 5 6 7 8 91 − − + + − − − + +2 − + + − − − + +3 − − + − + − −4 − + − + − −5 − − − + +6 − − − −7 − + +8 − −9 −The same nine mutants were then used in pairwise infections of E. coli B hosts. The production of progenyphages that can subsequently lyse E. coli K(λ) hosts isnow scored. In the table, 0 means the progeny do notproduce any plaques on E. coli K(λ) cells; − meansthat only a very few progeny phages produce plaques;and + means that many progeny produce plaques(more than 10 times as many as in the − cases).1 2 3 4 5 6 7 8 91 − + + + + − − + +2 − + + + + − + +3 0 − + 0 + + −4 − + − + + +5 − + − + +6 0 0 − +7 0 + +8 − +9 −a. Which of the mutants are the three deletions? Whatcriteria did…

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