D3S1358 vWA FGA Blood Stain 3000 2000 1000 15 2790 16 3075 1542 2500 Suspect 1 E900 600 는300 24 1033 29 16 Suspect 2 F2000 1000 10 23.2 2704 18 1942 600 818 Suspect 3 1500 -1000 -500 16 1629 1930 899 1187 778 Suspect 4 4000 -2000 16 16 10 24 5174 1980 1507 1220 17 1661
A blood stain from a crime scene and blood samples from four suspects were analyzed by PCR using fluorescent primers associated with three STR loci: D3S1358, vWA, and FGA. The resulting electrophoretograms are shown below. The numbers beneath each peak identify the allele (upper box) and the height of the peak in relative fluorescence units (lower box). Solve, (a) Since everyone has two copies of each chromosome and therefore, two alleles of each gene, what accounts for the appearance of
only one allele at some loci? (b) Which suspect is a possible source of the blood? (c) Could the suspect be identifi ed using just one of the three STR loci? (d) What can you conclude about the amount of DNA obtained from Suspect 1 compared to Suspect 4?
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