Create a Schematic Diagram from the Procedure:
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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Question
Create a Schematic Diagram from the Procedure:
PROCEDURE:
I. Isolation of Milk Component
A. Casein
- Pour 75ml of milk into a beaker and slowly add 35ml 10% lactic acid. Stir constantly until the Formation of casein precipitates.
- Filter and save the filtrate (filtrate 1) for procedure B & C.
- Collect the precipitate and place it into a beaker, add 25ml acetone.
- Stir well and filter the acetone. Catch the filtrate (filtrate2 for procedure D) in an evaporating dish.
- Transfer the casein to a filter paper and allow it to dry.
- Test the casein to prove that it is a protein (Proceed A.1Confirmatory Test for Casein Procedure)
A.1 Confirmatory Test for Casein
- Suspend the casein in 15ml distilled water
- Stir well and filter
- Perform Biuret, Millon’s, and Hopkins-Cole tests on the filtrate. (Refer Activity 9)
- Record Observation
B. Lactose
- From the filtrate (filtrate1) in procedure A, get 2ml sample.
- Perform Benedict’s Test.
C. Globulin
- To 5ml of the filtrate from procedure A, add 10 drops of phenolphthalein indicator, and then add milk of lime or a suspension of Ca(OH)2 until a faint pink color appears.
- Filter and make the filtrate neutral to litmus paper by adding 1% HCl solution.
- To this solution, add an equal volume of 10ml saturated (NH4)2SO4 solution
- Filter and note the results.
D. Fat
- Evaporate the acetone solution from procedure A.4 (filtrate2) in the evaporating dish to dryness on a steam bath. (Keep the evaporating dish away from the flame
- Observe the residue.
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