Consider you've done 3 pipetting trials for volumes 1000, 200, 20, and 2 using P2, P20, P200 and P1000. Do you think your dots will be the same across all trials? Why?
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Consider you've done 3 pipetting trials for volumes 1000, 200, 20, and 2 using P2, P20, P200 and P1000.
Do you think your dots will be the same across all trials? Why?
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- Same variants appeared in all patients analyzed with low coverage and Phred score (less then 20). What does it mean?How is 10^4 derived? Please show all calculation steps. # of cells per mL = (Average)*DF*10^4Interpret the two chromatograms below. Which of the two chromatograms will provide good sequencing data? Evaluate your answer using the following guide questions: How clear are the peaks? Do you observe baseline noise? Are there N calls or heterozygous peaks? Do peaks broaden?
- In STR typing, the homozygous condition will create a higher peak than the heterozygous condition in terms of relative fluorescence. How do the capillary electrophoresis and the electropherogram correlate?AH02You have a cell count of 6.7 x 10° cells / ml in a total volume of 4.5ml. You have resuspended your cells in 5ml sterile PBS. You need to seed a six-well plate with the following numbers: a. 2 wells at 1 x 10“ cells / well (2ml / well) b. 2 wells at 4 x 10 cells / well (2ml / well) c. 2 wells at 2 x 10° cells / well (2ml / well) Determine the volume of the original culture you will need to seed each concentration.
- This is my data collected from a Succinate Dehyrogenase Enzyme Assay - I am asked to plot a Concentration vs Time while plotting the Resuspended and Supernatant Reaction; how can this be completed? Test Tube Concentration (uM) Absorbance @ 600nm #1 0 0.00 #2 5 0.090 #3 10 0.187 #4 20 0.368 #5 40 0.715 #6 60 1.075 Table 3. Succinate Dehydrogenase Assay @ 600 nm Time point (min): Resuspended Pellet Reaction Absorbance Supernatant Reaction Absorbance Enzyme Reaction Control Absorbance 0 43.2 44.2 46.1 3 43.3 44.8 6 43.0 44.9 9 42.6 45.2 12 43.5 45.6 15 44.0 45.7 18 43.8 45.9 21 44.0 46.4 24 44.1 46.5 27 43.9 46.7 30 43.7 46.9 43.6Discuss the different designs for collecting the information desired in a Randomised Controlled Trial (RCT).Nicotinamide Adenine Dinucleotide (NAD) Assay (SIGMA Kit MAK037) analysis of tissue samples initially requires: centrifugation at 13,000g for 10min to homogenise tissue. centrifugation of 20mg of tissue at 2,000rpm with Extraction Buffer. freezing and thawing for 2 cycles of 20min before addition of Extraction Buffer. homogenization of PBS washed tissue with Extraction Buffer.
- A COVID-19 test, which produce result in 10 minutes, has the following test characteristics. The test has a 95% sensitivity and a 95% specificity. The State of Massachusetts is in dilemma whether to test symptomatic individuals or the entire residents to decrease the spread of infection as depicted in the following two scenarios. Scenario A. The State of Massachusetts wants to administer the test on 40,000 individuals who will develop cough, fever and shortness of breath in the next 10 days to identify COVID-19 infected individuals and institute public health measures. The estimated prevalence of COVID-19 in this population is 750 per 1,000 population using a gold-standard test. Scenario B. The State of Massachusetts wants to administer the test to its entire COVID-19 uninfected population (6,750,000). The estimated prevalence of COVID-19 in this population is 5 per 1,000 population using a gold-standard test. Please help the State of Massachusetts by responding to the…does P1000 and P5000 pass validation? Why, why not?Using the data in the photo of the two syringes below, answer these six questions: QUESTIONS 1. Which syringe had the most floating disks? Why? 2. What is the gas being released by the leaf disks? 3. Which syringe is the control? 4. Which syringe is the experimental? 5. What is the experimental variable in this experimental design? 6. List at least two controls in this experimental design.