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Consider the following DNA sample: 5' CCGAGAACTTA 3' Draw a gel showing the following: 1. Sanger sequencing (ddNTP's): a. Lane 1: A pre-made ladder with the following molecular weights: 2, 5, and 9 b.p. Draw and arrow and label each band for your ladder. b. Lane 2: PCR of sample using dGTP, dATP, dCTP, and dTTP c. Lane 3: PCR of sample using ddGTP, dATP, dCTP, and dTTP d. Lane 4: PCR of sample using dGTP, ddATP, dCTP, and dTTP e. Lane 5: PCR of sample using ddGTP, ddATP, ddCTP, and ddTTP

Consider the following DNA sample: 5' CCGAGAACTTA 3' Draw a gel showing the following: 1. Sanger sequencing (ddNTP's): a. Lane 1: A pre-made ladder with the following molecular weights: 2, 5, and 9 b.p. Draw and arrow and label each band for your ladder. b. Lane 2: PCR of sample using dGTP, dATP, dCTP, and dTTP c. Lane 3: PCR of sample using ddGTP, dATP, dCTP, and dTTP d. Lane 4: PCR of sample using dGTP, ddATP, dCTP, and dTTP e. Lane 5: PCR of sample using ddGTP, ddATP, ddCTP, and ddTTP

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Consider the following DNA sample: 5' CCGAGAACTTA 3' Draw a gel showing the following: 1. Sanger sequencing (ddNTP's): a. Lane 1: A pre-made ladder with the following molecular weights: 2, 5, and 9 b.p. Draw and arrow and label each band for your ladder. b. Lane 2: PCR of sample using dGTP, dATP, dCTP, and dTTP c. Lane 3: PCR of sample using ddGTP, dATP, dCTP, and dTTP d. Lane 4: PCR of sample using dGTP, ddATP, dCTP, and dTTP e. Lane 5: PCR of sample using ddGTP, ddATP, ddCTP, and ddTTP
Transcribed Image Text:Consider the following DNA sample: 5' CCGAGAACTTA 3' Draw a gel showing the following: 1. Sanger sequencing (ddNTP's): a. Lane 1: A pre-made ladder with the following molecular weights: 2, 5, and 9 b.p. Draw and arrow and label each band for your ladder. b. Lane 2: PCR of sample using dGTP, dATP, dCTP, and dTTP c. Lane 3: PCR of sample using ddGTP, dATP, dCTP, and dTTP d. Lane 4: PCR of sample using dGTP, ddATP, dCTP, and dTTP e. Lane 5: PCR of sample using ddGTP, ddATP, ddCTP, and ddTTP
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