Consider a mixture comprised of the following proteins below: Protein i MW (kDa) Charge (pH 7.4) Affinity to a specific ligand %Polarity A i 760 -3 None 45 300 +1 Carbohydrate 70 80 -1 None 60 D 55 +2 None 25 a. Protein that will most strongly bind to an anion exchange column: (Choices: A, B, C, D) b. Protein that will elute last in gel filtration chromatography: (Choices: A, B, C, D) c. Protein that will elute first in hydrophobic interaction chromatography: (Choices: A, B, C, D) d. Protein that will elute last in a carbohydrate containing column: (Choices: A, B, C, D)

Consider a mixture comprised of the following proteins below: Protein i MW (kDa) Charge (pH 7.4) Affinity to a specific ligand %Polarity A i 760 -3 None 45 300 +1 Carbohydrate 70 80 -1 None 60 D 55 +2 None 25 a. Protein that will most strongly bind to an anion exchange column: (Choices: A, B, C, D) b. Protein that will elute last in gel filtration chromatography: (Choices: A, B, C, D) c. Protein that will elute first in hydrophobic interaction chromatography: (Choices: A, B, C, D) d. Protein that will elute last in a carbohydrate containing column: (Choices: A, B, C, D)

Chemistry

10th Edition

ISBN:9781305957404

Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Chapter1: Chemical Foundations

Section: Chapter Questions

Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...

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