You are separating proteins on a cation exchange column. You need to be sure that the proteins are in a solution that is [Select] the pl values of the proteins. You can elute the proteins from the column by using a mobile phase gradient where the %NaCl in the eluent gradually [Select]

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Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
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You are separating proteins on a cation exchange column.  You need to be sure that the proteins are in a solution that is  [> 2.0 pH units below, or  0.5 - 1.5 pH units above or 0.5 - 1.5 pH units below, or
> 2.0 pH units above]  the pI values of the proteins.  You can elute the proteins from the column by using a mobile phase gradient where the %NaCl in the eluent gradually  ["increases, decreases"]  .  

You are separating proteins on a cation exchange column. You need to be sure that the proteins are
in a solution that is [Select ]
the pl values of the proteins. You can elute the
proteins from the column by using a mobile phase gradient where the %NaCl in the eluent gradually
[Select]
Transcribed Image Text:You are separating proteins on a cation exchange column. You need to be sure that the proteins are in a solution that is [Select ] the pl values of the proteins. You can elute the proteins from the column by using a mobile phase gradient where the %NaCl in the eluent gradually [Select]
Which type of chromatography would be the best choice for determining the molar mass
distribution in a sample of polyvinyl alcohol molecules?
Cation Exchange
Size exclusion
HPLC using a C-18 columnalse
Gas
Transcribed Image Text:Which type of chromatography would be the best choice for determining the molar mass distribution in a sample of polyvinyl alcohol molecules? Cation Exchange Size exclusion HPLC using a C-18 columnalse Gas
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