You are separating proteins on a cation exchange column. You need to be sure that the proteins are in a solution that is [Select] the pl values of the proteins. You can elute the proteins from the column by using a mobile phase gradient where the %NaCl in the eluent gradually [Select]

You are separating proteins on a cation exchange column. You need to be sure that the proteins are in a solution that is [Select] the pl values of the proteins. You can elute the proteins from the column by using a mobile phase gradient where the %NaCl in the eluent gradually [Select]

Principles of Modern Chemistry

8th Edition

ISBN:9781305079113

Author:David W. Oxtoby, H. Pat Gillis, Laurie J. Butler

Publisher:David W. Oxtoby, H. Pat Gillis, Laurie J. Butler

Chapter16: Solubility And Precipitation Equilibria

Section: Chapter Questions

Problem 52P

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Question

You are separating proteins on a cation exchange column. You need to be sure that the proteins are in a solution that is [> 2.0 pH units below, or 0.5 - 1.5 pH units above or 0.5 - 1.5 pH units below, or
> 2.0 pH units above] the pI values of the proteins. You can elute the proteins from the column by using a mobile phase gradient where the %NaCl in the eluent gradually ["increases, decreases"] .

You are separating proteins on a cation exchange column. You need to be sure that the proteins are
in a solution that is [Select ]
the pl values of the proteins. You can elute the
proteins from the column by using a mobile phase gradient where the %NaCl in the eluent gradually
[Select]

Which type of chromatography would be the best choice for determining the molar mass
distribution in a sample of polyvinyl alcohol molecules?
Cation Exchange
Size exclusion
HPLC using a C-18 columnalse
Gas

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