You are separating proteins on a cation exchange column. You need to be sure that the proteins are in a solution that is [Select] the pl values of the proteins. You can elute the proteins from the column by using a mobile phase gradient where the %NaCl in the eluent gradually [Select]
You are separating proteins on a cation exchange column. You need to be sure that the proteins are in a solution that is [Select] the pl values of the proteins. You can elute the proteins from the column by using a mobile phase gradient where the %NaCl in the eluent gradually [Select]
Chemistry
10th Edition
ISBN:9781305957404
Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Chapter1: Chemical Foundations
Section: Chapter Questions
Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...
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You are separating proteins on a cation exchange column. You need to be sure that the proteins are in a solution that is [> 2.0 pH units below, or 0.5 - 1.5 pH units above or 0.5 - 1.5 pH units below, or
> 2.0 pH units above] the pI values of the proteins. You can elute the proteins from the column by using a mobile phase gradient where the %NaCl in the eluent gradually ["increases, decreases"] .
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