Are the analyte molecules charged or not when isoelectric focusing separation is completed? Explain your answer. Explain the role of SDS in SDS-polyacrylamide gel electrophoresis (SDS-PAGE). State TWO factors that influence electrophoresis mobility.
Are the analyte molecules charged or not when isoelectric focusing separation is completed? Explain your answer. Explain the role of SDS in SDS-polyacrylamide gel electrophoresis (SDS-PAGE). State TWO factors that influence electrophoresis mobility.
Chemistry
10th Edition
ISBN:9781305957404
Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Chapter1: Chemical Foundations
Section: Chapter Questions
Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...
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Are the analyte molecules charged or not when isoelectric focusing separation is completed? Explain your answer.
Explain the role of SDS in SDS-polyacrylamide gel electrophoresis (SDS-PAGE).
State TWO factors that influence electrophoresis mobility.
Transcribed Image Text:(+) Low pH
(-)
(+)
(-) High pH
Isoelectric Focusing
SDS-PAGE
↑
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