We use reverse phase column chromatography with a non-polar C18 stationary phase and a mixture of acetonitrile and methanol as mobile phase in order to separate the dye molecules methyl fuchsin, parafuchsin, basic fuchsin and malachite green. What will be the order of elution of these molecules. Explain. Why won't these compounds separate using normal phase chromatography with silica and acetone?
We use reverse phase column chromatography with a non-polar C18 stationary phase and a mixture of acetonitrile and methanol as mobile phase in order to separate the dye molecules methyl fuchsin, parafuchsin, basic fuchsin and malachite green. What will be the order of elution of these molecules. Explain. Why won't these compounds separate using normal phase chromatography with silica and acetone?
Chemistry
10th Edition
ISBN:9781305957404
Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Chapter1: Chemical Foundations
Section: Chapter Questions
Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...
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We use reverse phase column chromatography with a non-polar C18 stationary phase and a mixture of acetonitrile and methanol as mobile phase in order to separate the dye molecules methyl fuchsin, parafuchsin, basic fuchsin and malachite green. What will be the order of elution of these molecules. Explain.
Why won't these compounds separate using normal phase chromatography with silica and acetone?
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