Chemistry
10th Edition
ISBN:9781305957404
Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Chapter1: Chemical Foundations
Section: Chapter Questions
Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...
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Question
- Briefly explain the process of ion-exchange chromatography.
Expert Solution
Step 1: Column Setup in Ion-Exchange Chromatography
Answer :----
Ion-exchange chromatography it begins by preparing a chromatography column filled with a solid, porous resin matrix.
Here, this resin is special because it contains the charged of functional groups that are covalently attached to the resin's surface.
Now, the choice of the resin is pivotal and it depends on the nature of the ions you intend to separate.
Here, anion-exchange resins possess the negatively charged groups, while cation-exchange resins have a positively charged groups.
Therefore, these charged groups play a very crucial role in attracting and retaining the ions during the separation process.
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