13. You were provided with a trypsin stock solution of known enzymatic concentration of 0.75 ug/ml. A small amount was taken and diluted by a factor of 5 in a separate test tube. You then took 2.5 ml out of the diluted trypsin and added it to a glass test tube containing the following reagents - 500 ul of water, 250 ul of cofactor and 1.75 ml of buffer. Calculate the final concentration of the trypsin in the glass test tube and express the answer in ug/ml.

13. You were provided with a trypsin stock solution of known enzymatic concentration of 0.75 ug/ml. A small amount was taken and diluted by a factor of 5 in a separate test tube. You then took 2.5 ml out of the diluted trypsin and added it to a glass test tube containing the following reagents - 500 ul of water, 250 ul of cofactor and 1.75 ml of buffer. Calculate the final concentration of the trypsin in the glass test tube and express the answer in ug/ml.

Chemistry

10th Edition

ISBN:9781305957404

Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Chapter1: Chemical Foundations

Section: Chapter Questions

Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...

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Spectroanalytical Methods

Spectroanalytical methods measure the wavelength and intensity of radiations that are either emitted or absorbed by a molecular or atomic species. These methods are useful for evaluating the electronic or atomic arrangement of the molecules of a given analyte.

Question

13. You were provided with a trypsin stock solution of known enzymatic
concentration of 0.75 ug/ml. A small amount was taken and diluted by a factor of 5
in a separate test tube. You then took 2.5 ml out of the diluted trypsin and added it
to a glass test tube containing the following reagents 500 ul of water, 250 ul of
cofactor and 1.75 ml of buffer. Calculate the final concentration of the trypsin in the
glass test tube and express the answer in ug/ml.

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