N-(2-hydroxyethyl)piperazine-N'-(2-ethanesulfonic A purified protein is in a Hepes acid) buffer at pH 7 with 375 mM NaCl. A dialysis membrane tube holds a 2.0 mL sample of the protein solution. The sample tube floats in a beaker containing 1.00 L of the same Hepes buffer, but with 0 mM NaCl, for dialysis. Small molecules and ions (such as Na+, CI, and Hepes) can to diffuse across the dialysis membrane, but the protein cannot. Assume there are no sample volume changes during the dialysis. Calculate the final concentration of NaCl in the protein sample once the dialysis has come to equilibrium. Calculate the final NaCl concentration in the 2.0 mL protein sample after dialysis in 150 mL of the same Hepes buffer, with 0 mM NaCl, twice in succession. [NaCl] after a single dialysis: [NaCl] after a double dialysis: x10' TOOLS mM mM
N-(2-hydroxyethyl)piperazine-N'-(2-ethanesulfonic A purified protein is in a Hepes acid) buffer at pH 7 with 375 mM NaCl. A dialysis membrane tube holds a 2.0 mL sample of the protein solution. The sample tube floats in a beaker containing 1.00 L of the same Hepes buffer, but with 0 mM NaCl, for dialysis. Small molecules and ions (such as Na+, CI, and Hepes) can to diffuse across the dialysis membrane, but the protein cannot. Assume there are no sample volume changes during the dialysis. Calculate the final concentration of NaCl in the protein sample once the dialysis has come to equilibrium. Calculate the final NaCl concentration in the 2.0 mL protein sample after dialysis in 150 mL of the same Hepes buffer, with 0 mM NaCl, twice in succession. [NaCl] after a single dialysis: [NaCl] after a double dialysis: x10' TOOLS mM mM
Chemistry
10th Edition
ISBN:9781305957404
Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
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Chapter1: Chemical Foundations
Section: Chapter Questions
Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...
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![N-(2-hydroxyethyl)piperazine-N'-(2-ethanesulfonic
A purified protein is in a Hepes
acid) buffer at pH 7 with 375 mM NaCl. A
dialysis membrane tube holds a 2.0 mL sample of the protein solution. The sample tube floats in a beaker containing 1.00 L of
the same Hepes buffer, but with 0 mM NaCl, for dialysis. Small molecules and ions (such as Na+, Cl, and Hepes) can to
diffuse across the dialysis membrane, but the protein cannot. Assume there are no sample volume changes during the dialysis.
Calculate the final concentration of NaCl in the protein
sample once the dialysis has come to equilibrium.
Calculate the final NaCl concentration in the 2.0 mL protein
sample after dialysis in 150 mL of the same Hepes buffer,
with 0 mM NaCl, twice in succession.
[NaC1] after a single dialysis:
[NaCl] after a double dialysis:
x10'
TOOLS
mM
mM](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2F801f936d-7b9d-451b-afe9-d37e8c252f0b%2Fb0d7d75b-9da8-4c24-a46a-45c4036b857b%2F6kns7n_processed.png&w=3840&q=75)
Transcribed Image Text:N-(2-hydroxyethyl)piperazine-N'-(2-ethanesulfonic
A purified protein is in a Hepes
acid) buffer at pH 7 with 375 mM NaCl. A
dialysis membrane tube holds a 2.0 mL sample of the protein solution. The sample tube floats in a beaker containing 1.00 L of
the same Hepes buffer, but with 0 mM NaCl, for dialysis. Small molecules and ions (such as Na+, Cl, and Hepes) can to
diffuse across the dialysis membrane, but the protein cannot. Assume there are no sample volume changes during the dialysis.
Calculate the final concentration of NaCl in the protein
sample once the dialysis has come to equilibrium.
Calculate the final NaCl concentration in the 2.0 mL protein
sample after dialysis in 150 mL of the same Hepes buffer,
with 0 mM NaCl, twice in succession.
[NaC1] after a single dialysis:
[NaCl] after a double dialysis:
x10'
TOOLS
mM
mM
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