11. A TLC plate is spotted with each of the three compounds. The plate is developed using hexanes: ethyl acetate (5:95) to give the chromatogram shown below. How could you change the solvent system to give better separation of these three compounds? 12. Arrange the following in order of increasing Ry on thin-layer chromatography analysis plate based on their structure and how they adsorp and separate on TLC plate: Octanoic acid, Butyraldehyde, 2- Octanone, Decane, and 1-pentanol. (Hint: Background information document: What separates the compounds as a chromatogram develops?) 13. You are trying to determine TLC solvent system which will separate the compounds X, Yand Z. you ran a TLC plate using hexane/ ethyl acetate 95:5 as the eluting solvent and obtained the chromatogram below. How would you change the solvent system to give better separtion of these three compounds? 00 XYZ

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Seperation of Amino Acids by thin layer chromotography Lab. Please answer #11, #12, and #13 as I am very confused. Thank you for your help!

11. A TLC plate is spotted with each of the three compounds. The plate is developed using hexanes:
ethyl acetate (5:95) to give the chromatogram shown below. How could you change the solvent system
to give better separation of these three compounds?
12. Arrange the following in order of increasing Rƒ on thin-layer chromatography analysis plate based on
their structure and how they adsorp and separate on TLC plate: Octanoic acid, Butyraldehyde, 2-
Octanone, Decane, and 1-pentanol. (Hint: Background information document: What separates the
compounds as a chromatogram develops?)
13. You are trying to determine TLC solvent system which will separate the compounds X, Yand Z. you
ran a TLC plate using hexane/ ethyl acetate 95:5 as the eluting solvent and obtained the chromatogram
below. How would you change the solvent system to give better separtion of these three compounds?
D
X Y Z
Transcribed Image Text:11. A TLC plate is spotted with each of the three compounds. The plate is developed using hexanes: ethyl acetate (5:95) to give the chromatogram shown below. How could you change the solvent system to give better separation of these three compounds? 12. Arrange the following in order of increasing Rƒ on thin-layer chromatography analysis plate based on their structure and how they adsorp and separate on TLC plate: Octanoic acid, Butyraldehyde, 2- Octanone, Decane, and 1-pentanol. (Hint: Background information document: What separates the compounds as a chromatogram develops?) 13. You are trying to determine TLC solvent system which will separate the compounds X, Yand Z. you ran a TLC plate using hexane/ ethyl acetate 95:5 as the eluting solvent and obtained the chromatogram below. How would you change the solvent system to give better separtion of these three compounds? D X Y Z
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