Cloning vector contains a multiple cloning site (MCS) or polylinker containing multiple unique restriction sites. It also contains markers that allow for rapid selection of colonies with insert-containing plasmids in them.     The 1st statement is True and the 2nd statement is False.     The 1st statement is False and the 2nd statement is True.     Both Staments are True.     Both statements are False.

Q: an insert check (labeled A-D below). After growing cultures, you isolate the plasmids and treat them…

A: In E. Coli lac operon contains a structural gene lac Z that codes for the enzyme beta galactosidase.…

Q: A biotech lab technician is working with a mutated form of a particular bacterial gene and wants to…

A: Plasmids  are naturally occurring extra chromosomal double standard DNA molecules that replicate…

Q: CRISPR-Cas9

A: Both statements are true.

Q: Provide 2 diagrams to illustrate the 2 potential recombinant plasmids,

A: The question here states about the concerned recombination of cloned plasmids and their…

Q: Sickle cell anaemia is caused by a mutation in the HBB gene. The normal wild-type βA allele contains…

A: Sickle cell anemia is a result of a mutation in the HBB gene. This gene contains the restriction…

Q: Statement 1: Restriction enzymes are sequence-specific, which means that they only recognize a short…

A: Restriction endonuclease is an important tool in DNA Recombinant technology or genetic engineering.…

Q: A PCR reaction was performed to amplify the CHE2 gene. The PCR primers used were designed to amplify…

A: Polymerase chain reaction is a molecular biology technique that is sued to synthesize multiple…

Q: Many resistance mechanisms are encoded on plasmids. These mechanisms are of great clinical…

A: BLAST is a bioinformatics tool that enables us to identify a gene if we know its DNA sequence or the…

Q: plate your cells on a master plate without any antibiotics.  After colonies appear, you pick and…

A: Recombinant DNA technology is helpful for different gene related experiments.

Q: A plasmid vector has two genes in it, a gene for streptomycin resistance (strR) and another for…

A: Introduction :- Recombinant DNA technology ( also known as genetic engineering) involves the use of…

Q: For a molecular cloning experiment using the bacterial strain E. coli K12 is used to insert the gene…

A: Recombinant DNA technology uses enzymes and a variety of laboratory procedures to modify and isolate…

Q: After transformation you were asked to grow bacterial cells transformed with plasmid on a plate that…

A: Bacterial transformation is a process through which bacteria can take up foreign DNA. Transformation…

Q: A plasmid vector has two genes in it, a gene for penicillin resistance (penR) and another for…

A: "Biotechnology" is the use of our knowledge of biological processes to the development of beneficial…

Q: position of the Start and Stop. The GFP and RFP genes shown do not have start

A: The Cre-Lox system is a widely used tool in genetic engineering for site-specific modifications in…

Q: What's the cloning process from the first step to the last? Put the steps in order based on the…

A: Cloning is a technique for creating a genetically identical copy of a cell or organism. It is a…

Q: A plasmid vector has two genes in it, a gene for streptomycin resistance (strR) and another for…

A: A plasmid vector is a small, circular DNA molecule that is commonly used in molecular biology for…

Q: Why are nucleic acid probes necessary to lyse cells containing plasmids in order to detect the…

A: Cell lysis is frequently the very first step in most purification techniques for nucleic acids,…

Q: Some expression plasmids have small peptide or protein tags in front of and/or after the cloning…

A: An expression plasmid or expression vector is a plasmid which is designed for gene expression in…

Q: When using a conventional plasmid cloning vector containing a b-galactosidase gene, it is possible…

A: The blue–white screen is a techinque that allows for the rapid and convenient detection of…

Q: The diagram below shows that of a plasmid containing genes that confers erythromycin (Erm®) and…

A: Plasmids are small circular double-stranded DNA molecules that store additional genetic information…

Q: A newly discovered insect has a genomic DNA that does not contain any repetitive DNA or any intronic…

A:

Q: plasmid has been modified such that it carries the gene for green fluorescent protein (GFP) under…

A: GFP protein is found in the photoorgans of Aequorea Victoria a jelly fish. It is a 28 kDa protein.…

Q: Differentiate between TWO of the following pairs: Genetic and a restriction map Southern and…

A: The mapping of DNA refers to the identification of the location of particular genes. Genetic map and…

Q: plasmid vector has two genes in it, a gene for streptomycin resistance (strR) and another for…

A:

Q: Ndel 183 Aatil 2617 Sspl 2501 Ehel 235 Pdml 2204 Bogl 2215 Scal 217 146/ 230 MCS lacz 2486 PUC18…

A: INTRODUCTION Screening techniques It inclued Blue white assay, Agarose gel electrophoresis, replica…

Q: Once cloning is complete, a recombinant OXA-M290 protein can be produced in E. coli. Competent E.…

A: The addition of IPTG to E. coli BL21(DE3) cells containing the pET-28a-OXA-M290 plasmid induces the…

Q: Besides the pUC series, there are other plasmid vectors in the market such as pBR327 and pGEM-T.…

A: Cloning methods are used in biotechnology for various purposes. TA cloning is the technique that…

Q: A plasmid vector has two genes in it, a gene for penicillin resistance (penR) and another for…

A: The plasmid vector with the inserted gene of interest is called a recombinant plasmid. The process…

Q: In Figure 5-19, how many different bacterial species areshown as having contributed DNA to the…

A: A plasmid is an extrachromosomal circular DNA molecule found in the bacteria which is able to…

Q: In a transformation experiment, a sample of competent bacteria was mixed with a plasmid containing…

A: In a bacterial colony some bacteria are resistant to an antibiotic and some are susceptible.…

Q: Margaret has been given a plasmid containing her favorite gene, afg (Margaret’s favorite gene). The…

A: First, convert the amount of plasmid from micrograms (μg) to nanograms (ng) since the concentration…

Q: the role (presence and absence) of a selectable marker (selection) in cloning a recombinant DNA…

A:

Q: Bacteria are the most common GMOs because their simple structure permits easy manipulation of their…

A: Recombinant DNA is a technique for cutting and pasting DNA sequences of interest using enzymes.

Q: You are working with a recA- strain of E. coli (cannot produce functional RecA, nor carry out…

A: Conjugation is a process in which the bacteria transfers a plasmid (F plasmids) through the…

Q: Please help urgently

A: Conjugation is the process that explain the reproduction module in various bacteria species.

Q: Describe the molecular features of a BAC cloning vector. What is theprimary advantage of a BAC…

A: Cloning is defined as the process of formation of the individuals possessing identical DNA. This…

Q: Find a plasmid map for pET11a and create a basic procedure for cloning a gene into this vector.…

A: The cloning includes the cloning vector. The bacteria with the recombinant plamid is grown in…

Q: A newly discovered insect has a genomic DNA that does not contain any repetitive DNA or any intronic…

A: Biotechnology is the use of our understanding of biological processes to develop useful applications…