Cloning vector contains a multiple cloning site (MCS) or polylinker containing multiple unique restriction sites. It also contains markers that allow for rapid selection of colonies with insert-containing plasmids in them.     The 1st statement is True and the 2nd statement is False.     The 1st statement is False and the 2nd statement is True.     Both Staments are True.     Both statements are False.

Q: Early gene-cloning experiments involved insertion at one restriction site in the vector; for…

A: Answer: Introduction: Restriction endonucleases enzymes which cut DNA at precise recognition sites.…

Q: Describe how restriction enzymes like EcoR1 are used to create recombinant plasmids and what the…

A: The next stage is called "isolation of target DNA," and it involves removing the DNA that contains…

Q: The table below shows restriction enzyme pairs used by various teams to digest the YFG and clone it…

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Q: A result of agarose gel electrophoresis of plasmid DNA is shown below. The direction of the…

A: When we isolate the plasmid it can be in relaxed, nicked or supercoiled forms. The supercoiled form…

Q: Now that you’ve isolated the gene and made lots of copies, you need to insert the gene into…

A: Plasmids are used in genetic engineering to increase the number of copies of a gene. A plasmid is a…

Q: As you know, restriction enzymes evolved in different bacterial species independently. The adaptive…

A: the A restriction enzyme, restriction endonuclease, or restricts is an enzyme that cleaves DNA into…

Q: A) Outline the experimental procedure for cloning a eukaryotic gene and expressing it in E. coli.…

A: Genetic engineering has enabled us to engineer the genes according to the desired gene product. It…

Q: After transformation you were asked to grow bacterial cells transformed with plasmid on a plate that…

A: Bacterial transformation is a process through which bacteria can take up foreign DNA. Transformation…

Q: С 17 Based on the knowledge you gained from the cloning module, which of the bands in the figure is…

A: Agarose gel electrophoresis is a method used in molecular biology to resolve DNA fragments on the…

Q: plasmid has been modified such that it carries the gene for green fluorescent protein (GFP) under…

A: GFP protein is found in the photoorgans of Aequorea Victoria a jelly fish. It is a 28 kDa protein.…

Q: Which of the following best explains the natural function to the tumor-inducing (Ti) plasmid? It…

A: The deoxyribonucleic acid (DNA) is the double-stranded molecule that is the genetic material in most…

Q: In cloning human DNA, why is it necessary to insert the DNA into a vector such as a bacterial…

A: Plasmid are small, circular, extra-chromosomal, double stranded DNA molecule. They are capable of…

Q: Margaret has been given a plasmid containing her favorite gene, afg (Margaret’s favorite gene). The…

A: First, convert the amount of plasmid from micrograms (μg) to nanograms (ng) since the concentration…

Q: The map of plasmid pUC19 is shown below.  The restriction site coordinate is the position of the…

A: Introduction Plasmid Vectors: these are like the artificial vehicle to deliver our gene of insert…

Q: The following statements are true about common gene cloning procedures except: -DNA plasmids can…

A: Biotechnology is a branch of biology, including the use of living organisms to produce products.…

Q: A newly discovered insect has a genomic DNA that does not contain any repetitive DNA or any intronic…

A: Biotechnology is the use of our understanding of biological processes to develop useful applications…

Q: Restriction enzymes are essential tools in genetic engineering. Discuss with diagrams

A: As per our guidelines, we are supposed to answer only one question. Kindly repost the other question…

Q: The genomic DNA of a bacterial cell is NOT destroyed by the cell’s own restriction enzymes because

A: Restriction enzymes Restriction enzyme or restrictions endonuclease are use to cleave DNA or RNA at…

Q: Restriction mapping of a linear piece of DNA reveals the following EcoRI restriction sites. EcoRI…

A: Introduction By cleaving the internal covalent bonds between nucleotides, endonucleases breaks down…

Q: You have set up a recombinant DNA experiment using the plasmid PBR322 as the vector (see plasmid…

A: PBR322 is a cloning vector which is widely used for cloning purposes. The map of PBR322 is given in…