CASE -1 The wicked queen in her search for youth extract came across a hepta-peptide (HP) she believes would be beneficial. Prior to trying it she ordered her house aids in the castle who happened to know biochemistry to identify the primary structure of the HP. She found that HP is composed of: D, L, K, M, F, & Y. Further investigation requested by the wicked queen involved: D= Asp; L=Leu; K=Lys; M= Met; F=Phe; Y=Tyr. 1. Treatment of HP with trypsin, which yielded no fragments indicting that A. HP has no amino acid that may be recognized by trypsin as a cleavage site B. HP is not cleavable by trypsin since arginine is not among the composing aa C. Tryptic cleavage site is present at the amino terminal of HP D. Most probably HP has a K residue residue at the C-terminus 2. Treatment of HP with CNBr yielded: a dipeptide, a tetrapeptide & a free K indicating that A. Amino acid M is at N terminal B. There are 1 M and 1 C residue C. There are 2 M residues with one preceding K D. Mand K are cleavage sites for CNBR 3. Treatment of HP with chymotrypsin yielded: a free amino acid, a dipeptide, and a tetrapeptide composed of: L, K, & M. The HP structure is: A. LMDFMLF B. KMLYMDF C. FDYMLMK D. MLLYDFF 4. Estimated net charge on the HP at pH 1.5 and 7.4 are respectively : A. +1 and -2 B. 2 and zero C. +2 and -2 D. -1 and zero
CASE -1 The wicked queen in her search for youth extract came across a hepta-peptide (HP) she believes would be beneficial. Prior to trying it she ordered her house aids in the castle who happened to know biochemistry to identify the primary structure of the HP. She found that HP is composed of: D, L, K, M, F, & Y. Further investigation requested by the wicked queen involved: D= Asp; L=Leu; K=Lys; M= Met; F=Phe; Y=Tyr. 1. Treatment of HP with trypsin, which yielded no fragments indicting that A. HP has no amino acid that may be recognized by trypsin as a cleavage site B. HP is not cleavable by trypsin since arginine is not among the composing aa C. Tryptic cleavage site is present at the amino terminal of HP D. Most probably HP has a K residue residue at the C-terminus 2. Treatment of HP with CNBr yielded: a dipeptide, a tetrapeptide & a free K indicating that A. Amino acid M is at N terminal B. There are 1 M and 1 C residue C. There are 2 M residues with one preceding K D. Mand K are cleavage sites for CNBR 3. Treatment of HP with chymotrypsin yielded: a free amino acid, a dipeptide, and a tetrapeptide composed of: L, K, & M. The HP structure is: A. LMDFMLF B. KMLYMDF C. FDYMLMK D. MLLYDFF 4. Estimated net charge on the HP at pH 1.5 and 7.4 are respectively : A. +1 and -2 B. 2 and zero C. +2 and -2 D. -1 and zero
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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Transcribed Image Text:CASE -1
The wicked queen in her search for youth extract came across a hepta-peptide (HP) she believes would
be beneficial. Prior to trying it she ordered her house aids in the castle who happened to know biochemistry
to identify the primary structure of the HP. She found that HP is composed of: D, L, K, M, F, & Y.
Further investigation requested by the wicked queen involved:
D= Asp; L=Leu; K=Lys; M= Met; F=Phe; Y=Tyr.
1. Treatment of HP with trypsin, which yielded no fragments indicting that
A. HP has no amino acid that may be recognized by trypsin as a cleavage site
B. HP is not cleavable by trypsin since arginine is not among the composing aa
C. Tryptic cleavage site is present at the amino terminal of HP
D. Most probably HP has a K residue residue at the C-terminus
2. Treatment of HP with CNBR yielded: a dipeptide, a tetrapeptide & a free K indicating that
de
A. Amino acid M is at N terminal
B. There are 1 M and 1 C residue
C. There are 2 M residues with one preceding K
D. M and K are cleavage sites for CNBR
3. Treatment of HP with chymotrypsin yielded: a free amino acid, a dipeptide, and a tetrapeptide
composed of: L, K, & M. The HP structure is:
A. LMDFMLF
B. KMLYMDF
C. FDYMLMK
D. MLLYDFF
4. Estimated net charge on the HP at pH 1.5 and 7.4 are respectively :
A. +1 and -2
B. 2 and zero
C. +2 and -2
D. -1 and zero
CASE - 2
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