Give answer all questions with explanation please 14. You have been given a liquid preparation of amixture of the molecules shown below. What techniquewould you use to separate the five components of themixture and what the expected resulted would be basedon the specific procedure you have used.Glycine,Arginine,Histidine,Glutamic acidAnd the Pentapeptide...ーc-CH-CHạN-CH-C-N-H.CH-C-OHCHCHCHCHHSNH-NHNH2OH

A polypeptide is made up of an amino acid chain.

mixture and what the expected resulted would be bas on the specific procedure you have used. Glycine, Arginine, Histidine, Glutamic acid And the Pentapeptide... HN CH 23 CHC-N- CH-C CH-C- CH-C SH CH NH NH NH2 OH

mixture and what the expected resulted would be bas on the specific procedure you have used. Glycine, Arginine, Histidine, Glutamic acid And the Pentapeptide... HN CH 23 CHC-N- CH-C CH-C- CH-C SH CH NH NH NH2 OH

Basic Clinical Laboratory Techniques 6E

6th Edition

ISBN:9781133893943

Author:ESTRIDGE

Publisher:ESTRIDGE

Chapter6: Basic Clinical Chemistry

Section6.1: Introduction To Clinical Chemistry

Problem 9RQ

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Concept explainers

Parasitic infections

A parasite is an organism that lives in the body of another organism and obtains food at the expense of the host. There are two kinds of parasites they are internal and external. An internal parasite lives inside another organism and is known as endoparasite. An external parasite lives on the body surface of the host and is known as an ectoparasite.

Question

Give answer all questions with explanation please

14. You have been given a liquid preparation of a
mixture of the molecules shown below. What technique
would you use to separate the five components of the
mixture and what the expected resulted would be based
on the specific procedure you have used.
Glycine,
Arginine,
Histidine,
Glutamic acid
And the Pentapeptide...
ーc
-CH-C
HạN-
CH-C-N-
H.
CH-C-OH
CH
CH
CH
CH
HS
NH
-NH
NH2
OH

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27. Draw palmitic acid, identify a, B and w carbons, write out its short hand notation 28. Examine the peptide sequence below and then answer the questions: H2N-Gly- Leu- Ala-Asp-Cys-Asn-Trp-lle-Ser-Phe-Lys-Cys-Arg-Pro-coOH a. Circle the asparagine residue b. A possible intramolecular disulfide bond can be formed within this peptide between which two residues? c. Circle one residue which has a positively charged side chain under pH 7.4 d. Circle one residue which has a negatively charged side chain under pH 7.4 e. Circle the residue which can be phosphorylated
VII. Analysis of a peptide antibiotic purified from a strain of Bacillus brevii resulted in the following significant information: Complete hydrolysis of the peptide yielded equimolar amounts of Leu, Orn, Phe, Pro, and Val Molecular weight of the peptide was estimated to be aroung 1,200 Da The peptide failed to undergo hydrolysis when treated with carboxypeptidase Partial hydrolysis and then chromatographic separation of products yielded the following di- and tripeptides: Leu-Phe, Phe-Pro, Orn-Leu, Val-Orn, Val-Orn-Leu, Phe-Pro-Val, Pro-Val-Orn Treament with dinitrofluorobenzene (DNFB) followed by complete hydrolysis yielded only free amino acids and the DNFB-derivatized ornithine at its side chain Using this set of information available to you, deduce the amino acid sequence of this peptide antibiotic. Write your final peptide sequence only using 3-letter abbreviations.
4
A mixture of Aspartic Acid (pl 2.98), Histidine (pl 7.59), Lysine (pl 9.74), Phenylalanine (pl 5.48) and Threonine (pl 6.53) are separated by cation exchange chromatography. What is the order of elution of these amino acids if you use gradient buffer system from pH 10 to pH 2. v First 1. Aspartic Acid v Second 2. Histidine v Third 3. Lysine 4. Phenylalanine v Fourth 5. Threonine v Fifth 6. No separation
2. Vasopressin, a substance produced by the human body that regulates blood volume and pressure, is a nonapeptide with the following amino acid sequence. Describe the results you would expect when vasopressin is tested with the following reagents. sort GLY-ARG-PRO-CYS-ASN-GLN-PHE-TYR-CYS test xanthoproteic Millon's Hopkins-Cole nitroprusside observation A 250 25d ZYN CZ, ZINTEG DST ZW
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Mr. Bibo analyzed a mixture of amino acids using thin layer chromatography where the stationary phase is a silica-coated plate. For the mobile phase, he used 50% ethyl acetate in hexane. The mixture contained three (3) amino acids whose structures are shown below. HO. HO NH2 HO, HO. NH2 NH2 Phenylalanine Threonine Aspartic acid a.) From the amino acids listed above, identify spots A, B, C, and D in the chromatogram below. BO CO b.) Threonine can be phosphorylated using an enzyme called protein kinase. The reaction is shown below. Given the same conditions Mr. Bibo used in his analysis, will the phosphorylated threonine have a higher, smaller, or the same Rf value compared to threonine? Explain in one sentence. АТР ADP CH3 CH3 Protein kinase он он NH2 NH2 Threonine Phosphorylated threonine
1. The R group of the amino acid lysine is: -CH2-CH2-CH2-CH2-NH2. Draw the structure of lysine, including the sites of ionization, under the following conditions: 16 NI a) strongly acidic solution; b) neutral solution; c) strongly basic solution Drifed them ard What are the results of the following test on lysine? c) nitroprusside a) ninhydrin; b) biuret;
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1. Amino acid analysis of the peptide gave the following residues: Asp Leu Lys Met Phe Tyr. The following facts were observed: Trypsin treatment had no effect. The phenylthiohydantoin released by Edman degradation was C-C-CH2. Brief chymotrypsin treatment yielded several products including a dipeptide and a tetrapeptide. The tetrapeptide contained Leu, Lys and Met is some order. Cyanogen bromide treatment afforded a dipeptide, a tetrapeptide and a free Lys. What is the amino acid sequence for this heptapeptide?
1. Complete the table below with information about the amino acids utilized in this pro- cedure. Remember that smaller amino acids will travel further and so will amino acids that are soluble in the solvent. Table 9-1. Amino Acids Procedure AMINO ACIDS Phenylalanine Aspartic Acid Leucine Proline matemps DRAW THE MOLECULAR STRUCTURE in POLARITY (IS IT POLAR OR NONPOLAR?) odme her basalu la MOLECULAR WEIGHT SIZE (RANK LARGEST = 4 AND SMALLEST = 1) SHOULD IT REACT WITH NINHYDRIN?
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