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Mr. Bibo analyzed a mixture of amino acids using thin layer chromatography where the stationary phase is a silica-coated plate. For the mobile phase, he used 50% ethyl acetate in hexane. The mixture contained three (3) amino acids whose structures are shown below. `OH но, HO `OH NH2 NH2 NH2 Phenylalanine Threonine Aspartic acid a.) From the amino acids listed above, identify spots A, B, C, and D in the chromatogram below. BO b.) Threonine can be phosphorylated using an enzyme called protein kinase. The reaction is show below. Given the same conditions Mr. Bibo used in his analysis, will the phosphorylated threonine have a higher, smaller, or the same Rf value compared to threonine? Explain in one sentence.

Mr. Bibo analyzed a mixture of amino acids using thin layer chromatography where the stationary phase is a silica-coated plate. For the mobile phase, he used 50% ethyl acetate in hexane. The mixture contained three (3) amino acids whose structures are shown below. `OH но, HO `OH NH2 NH2 NH2 Phenylalanine Threonine Aspartic acid a.) From the amino acids listed above, identify spots A, B, C, and D in the chromatogram below. BO b.) Threonine can be phosphorylated using an enzyme called protein kinase. The reaction is show below. Given the same conditions Mr. Bibo used in his analysis, will the phosphorylated threonine have a higher, smaller, or the same Rf value compared to threonine? Explain in one sentence.

Biochemistry
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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Mr. Bibo analyzed a mixture of amino acids using thin layer chromatography where the stationary phase is a silica-coated plate. For the mobile phase, he used 50% ethyl acetate in hexane. The mixture contained three (3) amino acids whose structures are shown below. HO. HO NH2 HO, HO. NH2 NH2 Phenylalanine Threonine Aspartic acid a.) From the amino acids listed above, identify spots A, B, C, and D in the chromatogram below. BO CO b.) Threonine can be phosphorylated using an enzyme called protein kinase. The reaction is shown below. Given the same conditions Mr. Bibo used in his analysis, will the phosphorylated threonine have a higher, smaller, or the same Rf value compared to threonine? Explain in one sentence. АТР ADP CH3 CH3 Protein kinase он он NH2 NH2 Threonine Phosphorylated threonine
Transcribed Image Text:Mr. Bibo analyzed a mixture of amino acids using thin layer chromatography where the stationary phase is a silica-coated plate. For the mobile phase, he used 50% ethyl acetate in hexane. The mixture contained three (3) amino acids whose structures are shown below. HO. HO NH2 HO, HO. NH2 NH2 Phenylalanine Threonine Aspartic acid a.) From the amino acids listed above, identify spots A, B, C, and D in the chromatogram below. BO CO b.) Threonine can be phosphorylated using an enzyme called protein kinase. The reaction is shown below. Given the same conditions Mr. Bibo used in his analysis, will the phosphorylated threonine have a higher, smaller, or the same Rf value compared to threonine? Explain in one sentence. АТР ADP CH3 CH3 Protein kinase он он NH2 NH2 Threonine Phosphorylated threonine
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