A plot of V₁ on the y-axis versus V₁/ [S] on the x-axis is known as an Eadie-Hofstee plot. Like the Lineweaver-Burk plot, the Eadie-Hofstee plot is derived from a transformation of the Michaelis-Menten equation. The Michaelis-Menten equation expresses the relationship between the following terms: Vo, the rate of reaction; • Vmax, the maximal rate; [S], the substrate concentration; and KM, the Michaelis constant. Rearrange the Michaelis-Menten equation to obtain the Eadie-Hofstee equation, giving Vo as a function of Vo/[S]. ● In an Eadie-Hofstee plot, what is the significance of the x-intercept? Vmax/KM Vmax 1/[S] O-KM The Eadie-Hofstee plot illustrated shows enzyme activity in the absence of an inhibitor. Adjust one or both points so that the line shows enzyme activity in the presence of a competitive inhibitor. 20 19 18 17 16 15 14 13 12 11 10ܘܢ 9
A plot of V₁ on the y-axis versus V₁/ [S] on the x-axis is known as an Eadie-Hofstee plot. Like the Lineweaver-Burk plot, the Eadie-Hofstee plot is derived from a transformation of the Michaelis-Menten equation. The Michaelis-Menten equation expresses the relationship between the following terms: Vo, the rate of reaction; • Vmax, the maximal rate; [S], the substrate concentration; and KM, the Michaelis constant. Rearrange the Michaelis-Menten equation to obtain the Eadie-Hofstee equation, giving Vo as a function of Vo/[S]. ● In an Eadie-Hofstee plot, what is the significance of the x-intercept? Vmax/KM Vmax 1/[S] O-KM The Eadie-Hofstee plot illustrated shows enzyme activity in the absence of an inhibitor. Adjust one or both points so that the line shows enzyme activity in the presence of a competitive inhibitor. 20 19 18 17 16 15 14 13 12 11 10ܘܢ 9
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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C7 Q12:
![The Eadie-Hofstee plot illustrated shows enzyme activity in the absence of an inhibitor.
Adjust one or both points so that the line shows enzyme activity in the presence of a competitive inhibitor.
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V
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[S]](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2Fc94cb5d1-997a-49d0-b0ef-9ec02bf0a187%2F2c838fe5-c2e9-42d1-82f2-b9cfc8ab0a43%2Fsy7l8ze_processed.png&w=3840&q=75)
Transcribed Image Text:The Eadie-Hofstee plot illustrated shows enzyme activity in the absence of an inhibitor.
Adjust one or both points so that the line shows enzyme activity in the presence of a competitive inhibitor.
20
19
18
17
16
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[S]
![A plot of V₁ on the y-axis versus V₁/ [S] on the x-axis is known as an Eadie-Hofstee plot. Like the Lineweaver-Burk plot,
the Eadie-Hofstee plot is derived from a transformation of the Michaelis-Menten equation. The Michaelis-Menten
equation expresses the relationship between the following terms:
Vo, the rate of reaction;
• Vmax, the maximal rate;
[S], the substrate concentration; and
KM, the Michaelis constant.
Rearrange the Michaelis-Menten equation to obtain the Eadie-Hofstee equation, giving Vo as a function of Vo/[S].
●
In an Eadie-Hofstee plot, what is the significance of the x-intercept?
Vmax/KM
Vmax
1/[S]
O-KM
The Eadie-Hofstee plot illustrated shows enzyme activity in the absence of an inhibitor.
Adjust one or both points so that the line shows enzyme activity in the presence of a competitive inhibitor.
20
19
18
17
16
15
14
13
12
11
10ܘܢ
9](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2Fc94cb5d1-997a-49d0-b0ef-9ec02bf0a187%2F2c838fe5-c2e9-42d1-82f2-b9cfc8ab0a43%2Fuh4t7gg_processed.png&w=3840&q=75)
Transcribed Image Text:A plot of V₁ on the y-axis versus V₁/ [S] on the x-axis is known as an Eadie-Hofstee plot. Like the Lineweaver-Burk plot,
the Eadie-Hofstee plot is derived from a transformation of the Michaelis-Menten equation. The Michaelis-Menten
equation expresses the relationship between the following terms:
Vo, the rate of reaction;
• Vmax, the maximal rate;
[S], the substrate concentration; and
KM, the Michaelis constant.
Rearrange the Michaelis-Menten equation to obtain the Eadie-Hofstee equation, giving Vo as a function of Vo/[S].
●
In an Eadie-Hofstee plot, what is the significance of the x-intercept?
Vmax/KM
Vmax
1/[S]
O-KM
The Eadie-Hofstee plot illustrated shows enzyme activity in the absence of an inhibitor.
Adjust one or both points so that the line shows enzyme activity in the presence of a competitive inhibitor.
20
19
18
17
16
15
14
13
12
11
10ܘܢ
9
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