A plot of V₁ on the y-axis versus V₁/ [S] on the x-axis is known as an Eadie-Hofstee plot. Like the Lineweaver-Burk plot, the Eadie-Hofstee plot is derived from a transformation of the Michaelis-Menten equation. The Michaelis-Menten equation expresses the relationship between the following terms: Vo, the rate of reaction; • Vmax, the maximal rate; [S], the substrate concentration; and KM, the Michaelis constant. Rearrange the Michaelis-Menten equation to obtain the Eadie-Hofstee equation, giving Vo as a function of Vo/[S]. ● In an Eadie-Hofstee plot, what is the significance of the x-intercept? Vmax/KM Vmax 1/[S] O-KM The Eadie-Hofstee plot illustrated shows enzyme activity in the absence of an inhibitor. Adjust one or both points so that the line shows enzyme activity in the presence of a competitive inhibitor. 20 19 18 17 16 15 14 13 12 11 10ܘܢ 9
A plot of V₁ on the y-axis versus V₁/ [S] on the x-axis is known as an Eadie-Hofstee plot. Like the Lineweaver-Burk plot, the Eadie-Hofstee plot is derived from a transformation of the Michaelis-Menten equation. The Michaelis-Menten equation expresses the relationship between the following terms: Vo, the rate of reaction; • Vmax, the maximal rate; [S], the substrate concentration; and KM, the Michaelis constant. Rearrange the Michaelis-Menten equation to obtain the Eadie-Hofstee equation, giving Vo as a function of Vo/[S]. ● In an Eadie-Hofstee plot, what is the significance of the x-intercept? Vmax/KM Vmax 1/[S] O-KM The Eadie-Hofstee plot illustrated shows enzyme activity in the absence of an inhibitor. Adjust one or both points so that the line shows enzyme activity in the presence of a competitive inhibitor. 20 19 18 17 16 15 14 13 12 11 10ܘܢ 9
Principles Of Radiographic Imaging: An Art And A Science
6th Edition
ISBN:9781337711067
Author:Richard R. Carlton, Arlene M. Adler, Vesna Balac
Publisher:Richard R. Carlton, Arlene M. Adler, Vesna Balac
Chapter1: Basic Mathematics
Section: Chapter Questions
Problem 7RQ
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