Can you please interpret and discuss and mark, this gel electrophoresis results.  DNA ladder sizes: Band1: 23130, Band2: 9416, Band3: 6557, Band4: 4361, Band5: 2322, Band6: 564, Band7: 125

Q: a) Would you expect to find the largest or shortest DNA fragments closest to the negative end at the…

A: Gel electrophoresis is used to categorize and quantify proteins and nucleic acids. It is frequently…

Q: Choose one product of recominbinant DNA technology and write the steps involved in the production of…

A: The Flavr Savr was the first commercially produced genetically modified food to receive a human…

Q: Eco01091 2074 Pfol 46 BstAPI 170 Aatil 2017 Sspl 2501 Ndel 183 Ehel 235 Pdml 2204, Bogi 2215 Soal…

A: Restriction endonucleases also referred to as restriction enzymes are enzymes that cut DNA at…

Q: Explanation of TPO.

A: TPO=Thrombopoietin

Q: Outline the major steps invalved in recombinant DNA technalogy required to clane a gene into a…

A: INTRODUCTION Recombinant DNA technology In recombinant DNA technology we insert our gene of interest…

Q: How is the color of the spot coverted into useful data if data is collected from a Vicrochip DNA…

A: DNA microarray is a chip-based technique where a collection of microscopic DNA spots attached to a…

Q: What is the relationship of adherent cells and trypsin in Animal cell culture?

A: Trypsinization  is the process of cell dissociation using trypsin, a proteolytic enzyme which breaks…

Q: Identify types of plasmid( nicked, linear, supercolied and circular,stranded on each band

A: Answer: PLASMID DNA : It is the small, extrachromosomal DNA found in most bacteria and archea. It is…

Q: a PCR-based crime scene investigation, similar to the one presented in the lab module with Brother Y…

A: DNA sample taken from a crime scene is compared with a DNA sample from a suspect. If the two DNA…

Q: Determine the chemical reagents utilized in banana DNA extraction and their roles. What role does…

A: 3. Liquid dishwashing soap This helps split apart the cell membrane made of lipids and the cell…

Q: What benefits are there when using a DNA microarray over a genetic marker such as a STR?

A: The term genetic refers to the DNA molecule present in the nucleus of cells. DNA or deoxyribonucleic…

Q: BOTTOM The sequence depicted on the gel is 5'-GTGATGTAG-3' The sequence depicted on the gel is 3'-…

A: This method is used for DNA sequencing and it relies on the use of Deoxyribonucleoside…

Q: How can someone distribute the DNA in the spots if they are producing a Vicrochip DNA microarray and…

A: the microarray technology [MAT] uses the simple concept of hybridization via complementary…

Q: Why are entire genomes not used for DNA profiling?

A: DNA profiling is the process of characterizing a sequence of DNA obtained from a person. It is also…

Q: Nikolas is investigating the presence of four different genes (A, B, C and D) in Ladybugs using a…

A: PCR or Polymerase Chain Reaction is a technique which is used to create several copies of a…

Q: The figure below represents the size of various SSRS that are used for forensic analysis. The bars…

A: vWA produce 200bp in PCR . All others produce less or more than 200 bp in PCR.  Here SSR means is…

Q: A PCR reaction was performed to amplify the CHE2 gene. The PCR primers used were designed to amplify…

A: Polymerase chain reaction is a molecular biology technique that is sued to synthesize multiple…

Q: You are studying the DNA binding protein CLAMP and you want to determine its binding affinity for…

A: Introduction DNA acts as genetic material in our body. It is a double stranded molecule. mRNA is…

Q: After properly troubleshooting, you finally are able to visualize the gel. You obtained the image…

A: DNA extraction is a protocol of isolating genomic DNA from any cell type. Generally we use the…

Q: What is the fastest method to determine if a genetic disorder is due to a mutation at a palindromic…

A: Mutations This refers to the alterations in the sequence of DNA. These are caused from the mistakes…

Q: The following figure shows the FBI-style analysis of the genomic DNA of 10 people (1-10), and also…

A: Chromosomes are carrier of deoxyribonucleic acid (DNA). DNA is the genetic material. Simple sequence…

Q: Chironomus genomic DNA contains approximately 35.5% A. Estimate the percentages of G, C, and T in…

A: The double (ds) stranded DNA duplex structure follows the chargaffs rule, which suggests that the A…

Q: Why is it possible to visualize a PCR product on an agarose gel even if the template genome is…

A: Gel electrophoresis is a technique used in the separation of macromolecules such as proteins and DNA…

Q: The following plasmid is digested with EcoR1 and Ndel restriction endonucleases. Agarose…

A: Restriction endonucleases are enzymes which cleave the phosphodiester bonds within a DNA. These…

Q: What is the purpose of adding dishwashing liquid into the banana mixture? What reagent is used in…

A: DNA extraction is the method of isolation of genetic material ( DNA ) from plants , animals or any…

Q: After DNA fragments have been separated by gel electrophoresis, how can they be visualized?

A: Gel electrophoresis is a technique used for separating Deoxy ribonucleic acid (DNA) fragments based…

Q: DNA from 100 unrelated individuals from one population of Chinook salmon were amplified at a single…

A: A population is said to be in Hardy Weinberg equilibrium if it satisfies 2 equations; p+q+r=1 and…

Q: Regarding STR markers used in forensic science. Tick all the correct statements: no correct…

A: Short tandem repeats (STRs), which make up around 3% of the human genome, are  brief repeating DNA…

Q: Using the table below, what volume from the master mix will be dispensed to each sample tube if the…

A: Answer:  As we know, C1V1= C2V2, where C1 and C2 = initial and final concentrations V1 and V2 =…

Q: A PCR sample was loaded on an agarose gel for DNA electrophoresis. After completion, a single band…

A: From the graph on the right side The X-axis indicates migration distance in mmY axis indicates DNA…

Q: Use the gel to answer the following questions. You will be constructing a map of the plasmid,…

A: Restriction enzymes These are proteins that are isolated from the bacteria that cleave the sequences…

Q: Lane 1 = TakaraBio 200 bp DNA ladder Lane 2 = Haell only; (thick band) 1883 bp and 1871 bp, 799 bp…

A: Understanding the restriction digestion patterns of any plasmid is very important to know where…

Q: What are sticky ends? State their significance in recombination DNA technology.

A: Recombinant DNA Technology is a method for creating artificial DNA by combining genetic resources…

Q: Prior to submitting your candidate's DNA for Sanger sequencing, you performed a colony PCR reaction…

A: Sanger sequencing is a very important technique and needs to perform with certain protocols. If we…

Q: 22 22 22 16 22 7. Black Blue Purple Orange Green Gray | | | | || || || | || || || | || 8. | | || 7.…

A: Simple sequence repeats (SSRs) are also called microsatellite VNTR (variable number tandem repeat).…

Q: What information can be gathered by using DNA gel electrophoresis? Describe the steps in the process…

A: Gel electrophoresis is a molecular biology technique heavily used in laboratories. Electrophoresis…

Q: HI Molecule 2 Molecule 3 Legend: Thick black line - RNA. Thin black line - DNA. Thin dashed line -…

A: The polymerase chain reaction was given in 1985 by " Kary Mullis". It is a rapid in-vitro molecular…

Q: In a PCR-based crime scene investigation, similar to the one presented in the lab module with…

A: The RAPD or rapid amplification of polymorphic DNA is used to detect the DNA that is present in the…

Q: What will happen to the concentration and the A260/280 ratio of the DNA sample in the following…

A: Biotechnology is the use of our understanding of biological processes to develop useful applications…

Q: EcoRI 290 bp 3800 bp EcoRI PUC19 PUC19 3400 bp Bgll EcoRI 290 bp EcoRI 1900 bp PUC19 1500 bp

A: A plasmid circular, small, extra chromosomal double stranded DNA (dsDNA). pUC19 plasmid is one the…

Q: The production in a pcr reaction is: nucleotids double strand circular dna double stranf linear…

A: PCR stands for polymerase chain reaction.

Q: Which is the odd one out ? For the rest, explain the concept/process/technique they are involved…

A: DNA Polymerase is the main enzyme of replication. It adds deoxyribonucleotides according to the…

Q: Which of the following is/are true regarding a PCR reaction performed to amplify a DNA plasmid?…

A: PCR or polymerase chain reaction is a molecular technique which is used to quickly multiply a given…

Q: What is the purpose of adding the dishwashing liquid to the DNA extraction?

A: DNA extraction is a method of separating DNA from cell membranes, proteins, and other cellular…

Crime Scene Investigation

A crime scene refers to a place where a crime has occurred. The purpose of crime scene investigation (CSI) is to analyze the complete scene and catch the culprit based on the pieces of evidence collected from the place of crime. The people involved in this process are referred to as crime scene investigators.