Can you please interpret and discuss and mark, this gel electrophoresis results.  DNA ladder sizes: Band1: 23130, Band2: 9416, Band3: 6557, Band4: 4361, Band5: 2322, Band6: 564, Band7: 125

Q: a) Would you expect to find the largest or shortest DNA fragments closest to the negative end at the…

A: Gel electrophoresis is used to categorize and quantify proteins and nucleic acids. It is frequently…

Q: Choose one product of recominbinant DNA technology and write the steps involved in the production of…

A: The Flavr Savr was the first commercially produced genetically modified food to receive a human…

Q: Outline the major steps invalved in recombinant DNA technalogy required to clane a gene into a…

A: INTRODUCTION Recombinant DNA technology In recombinant DNA technology we insert our gene of interest…

Q: What is the probability of finding a 6 base pair palindrome in a sample of DNA? Answers A-E A 1/4 B…

A: Introduction  A palindrome sequence is a sequence of nucleotides (DNA) or amino acids (proteins)…

Q: How is the color of the spot coverted into useful data if data is collected from a Vicrochip DNA…

A: DNA microarray is a chip-based technique where a collection of microscopic DNA spots attached to a…

Q: Identify types of plasmid( nicked, linear, supercolied and circular,stranded on each band

A: Answer: PLASMID DNA : It is the small, extrachromosomal DNA found in most bacteria and archea. It is…

Q: What are STRs, and why are they useful for DNA profiling?

A: Introduction  In the genome there are usually two type of nucleic acid sequence present. One is Non…

Q: a PCR-based crime scene investigation, similar to the one presented in the lab module with Brother Y…

A: DNA sample taken from a crime scene is compared with a DNA sample from a suspect. If the two DNA…

Q: Determine the chemical reagents utilized in banana DNA extraction and their roles. What role does…

A: 3. Liquid dishwashing soap This helps split apart the cell membrane made of lipids and the cell…

Q: (b) Use a drawing to illustrate the principle of DNA gel electrophoresis.

A: Gel electrophoresis is the technique of separation of molecules on the basis of their size and…

Q: BOTTOM The sequence depicted on the gel is 5'-GTGATGTAG-3' The sequence depicted on the gel is 3'-…

A: This method is used for DNA sequencing and it relies on the use of Deoxyribonucleoside…

Q: How can someone distribute the DNA in the spots if they are producing a Vicrochip DNA microarray and…

A: the microarray technology [MAT] uses the simple concept of hybridization via complementary…

Q: Why are entire genomes not used for DNA profiling?

A: DNA profiling is the process of characterizing a sequence of DNA obtained from a person. It is also…

Q: After properly troubleshooting, you finally are able to visualize the gel. You obtained the image…

A: DNA extraction is a protocol of isolating genomic DNA from any cell type. Generally we use the…

Q: What is the fastest method to determine if a genetic disorder is due to a mutation at a palindromic…

A: Mutations This refers to the alterations in the sequence of DNA. These are caused from the mistakes…

Q: The following figure shows the FBI-style analysis of the genomic DNA of 10 people (1-10), and also…

A: Chromosomes are carrier of deoxyribonucleic acid (DNA). DNA is the genetic material. Simple sequence…

Q: Chironomus genomic DNA contains approximately 35.5% A. Estimate the percentages of G, C, and T in…

A: The double (ds) stranded DNA duplex structure follows the chargaffs rule, which suggests that the A…

Q: What is the purpose of adding dishwashing liquid into the banana mixture? What reagent is used in…

A: DNA extraction is the method of isolation of genetic material ( DNA ) from plants , animals or any…

Q: After DNA fragments have been separated by gel electrophoresis, how can they be visualized?

A: Gel electrophoresis is a technique used for separating Deoxy ribonucleic acid (DNA) fragments based…

Q: Mingyu isolated double-stranded (ds) DNA from Matricaria chamomilla, a medicinal plant that is used…

A: Volume of ds-DNA = 2.50 mLVolume of distilled water = 12.00 mLActual concentration of ds DNA =…

Q: Lane 1 = TakaraBio 200 bp DNA ladder Lane 2 = Haell only; (thick band) 1883 bp and 1871 bp, 799 bp…

A: Understanding the restriction digestion patterns of any plasmid is very important to know where…

Q: HI Molecule 2 Molecule 3 Legend: Thick black line - RNA. Thin black line - DNA. Thin dashed line -…

A: The polymerase chain reaction was given in 1985 by " Kary Mullis". It is a rapid in-vitro molecular…

Q: In a PCR-based crime scene investigation, similar to the one presented in the lab module with…

A: The RAPD or rapid amplification of polymorphic DNA is used to detect the DNA that is present in the…

Q: What will happen to the concentration and the A260/280 ratio of the DNA sample in the following…

A: Biotechnology is the use of our understanding of biological processes to develop useful applications…

Q: EcoRI 290 bp 3800 bp EcoRI PUC19 PUC19 3400 bp Bgll EcoRI 290 bp EcoRI 1900 bp PUC19 1500 bp

A: A plasmid circular, small, extra chromosomal double stranded DNA (dsDNA). pUC19 plasmid is one the…

Q: The production in a pcr reaction is: nucleotids double strand circular dna double stranf linear…

A: PCR stands for polymerase chain reaction.

Q: What is the sequence of the sample DNA submitted for sequencing given the gel electrophoresis…

A: Sanger sequencing method is chain terminating method.

Q: Based on the following image: A) Identify the largest DNA fragment in sample 5. Explain your…

A: In the given question, an agarose gel is shown which is having bands of DNA in different lanes. DNA…

Q: Labelled primers or dNTPs are useful in some recombinant DNA techniques. What are DNA primers? What…

A: 1* DNA primers is a short and single stranded nucleic acid sequence that is the starting point for…

Q: Identify 2 or more DNA-based technologies and discuss their combined applications in…

A: We need to discuss the combined uses of two technologies in the generation/development of a DNA…

Q: Are any of the loci on the X chromosome? If so, identify this (these) locus (loci) by color.

A: The term SSR stands for simple sequence repeat that is a microsatellite VNTR (variable number tandem…

Q: Why RT-PCR is important in the sample preparation to perform expression microarray experiment?

A: RT - PCR : Reverse transcription- polymerase chain reaction is one of the types of PCR.  DNA…

Q: The gel below shows results for the bitter tasting SNP analysis. Analyze the results and annotate…

A: PCR or polymerase chain reaction is a technique that is used to amplify the desired segment of DNA…

Q: Lane M 1 2 3 4 5 Content DNA ladder Replicate 1 Replicate 2 Replicate 3 Negative control Positive…

A: PCR stands for polymerase chain reaction. PCR is used to amplify nucleic acid segments for further…

Q: Which of the following statements is TRUE for DNA extraction? DNA yield and purity are inversely…

A: DNA extraction is a fundamental laboratory technique used to isolate DNA from cells or tissues for…

Q: Complete this Master Mix table for 8 DNA samples, a positive control, negative control, and an extra…

A: Volume per tube (uL) number of reactions volume in master mix (uL) PCR buffer 1.2 11 13.2 dNTP…

Q: What is the probability that any random male in USA would share the same genotype as the murderer…

A: SSR (simple sequence repeats) or microsatellite refers to a tract of repetitive co-dominant…

Q: Briefly explain the functions of the four procedures you learned about in this lab:   DNA…

A: DNA Extraction- DNA extraction is a technique to separate DNA from cell membranes, proteins, and…

Crime Scene Investigation

A crime scene refers to a place where a crime has occurred. The purpose of crime scene investigation (CSI) is to analyze the complete scene and catch the culprit based on the pieces of evidence collected from the place of crime. The people involved in this process are referred to as crime scene investigators.