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overlapping one another and amplifying themselves. Where do you expect to see bands of Primer dimer and/or RNA, if present, on your gel? A. approximately 50bp and 121bp fragment of the marker B. below 121bp fragment of the marker C. between 121-383bp fragments of the marker D. none of these

overlapping one another and amplifying themselves. Where do you expect to see bands of Primer dimer and/or RNA, if present, on your gel? A. approximately 50bp and 121bp fragment of the marker B. below 121bp fragment of the marker C. between 121-383bp fragments of the marker D. none of these

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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It is common to see one or two diffuse (fuzzy) bands of RNA and/or primer dimer at the bottom of the gel after PCR amplification. RNA may be found at approximately the position of the smallest fragment of the PBR322/BstNI marker you used alongside your samples you loaded. RNA is the largest component of nucleic acid isolated from plant tissue, so some RNA may escape digestion by the RNase incorporated into the TE buffer in Part Il of the experiment. Primer dimer is an artifact of the PCR reaction that results from two primers overlapping one another and amplifying themselves. Where do you expect to see bands of Primer dimer and/or RNA, if present, on your gel? A. approximately 50bp and 121bp fragment of the marker B. below 121bp fragment of the marker C. between 121-383bp fragments of the marker D. none of these
Transcribed Image Text:It is common to see one or two diffuse (fuzzy) bands of RNA and/or primer dimer at the bottom of the gel after PCR amplification. RNA may be found at approximately the position of the smallest fragment of the PBR322/BstNI marker you used alongside your samples you loaded. RNA is the largest component of nucleic acid isolated from plant tissue, so some RNA may escape digestion by the RNase incorporated into the TE buffer in Part Il of the experiment. Primer dimer is an artifact of the PCR reaction that results from two primers overlapping one another and amplifying themselves. Where do you expect to see bands of Primer dimer and/or RNA, if present, on your gel? A. approximately 50bp and 121bp fragment of the marker B. below 121bp fragment of the marker C. between 121-383bp fragments of the marker D. none of these
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