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Lane M 1 2 3 4 5 Content DNA ladder Replicate 1 Replicate 2 Replicate 3 Negative control Positive control 700 bp 600 bp M 1 2 3 4 5

Lane M 1 2 3 4 5 Content DNA ladder Replicate 1 Replicate 2 Replicate 3 Negative control Positive control 700 bp 600 bp M 1 2 3 4 5

Biochemistry
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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  1. Ben used the following primer pairs to amplify the COI region of his DNA template:

Afterwards, he used Agarose Gel Electrophoresis to visualize the PCR products.

Based on the gel:

a) Was he able to successfully amplify the desired region in all replicates?

b) Are there errors in Ben's gel? Does he need to repeat his PCR? 

 
Primer Set Universal Fish Primer name Fish_Univ_ F Fish_Univ_R Direction Forward Reverse Primer sequence (5'-3') CACGACGTTGTAAAACGACACYAAICAYAAAGAYATIGGCAC GGATAACAATTICACACAGGACITCAGGGTGWCCGAARAAYCARAA Barcode length (bp) 652
Transcribed Image Text:Primer Set Universal Fish Primer name Fish_Univ_ F Fish_Univ_R Direction Forward Reverse Primer sequence (5'-3') CACGACGTTGTAAAACGACACYAAICAYAAAGAYATIGGCAC GGATAACAATTICACACAGGACITCAGGGTGWCCGAARAAYCARAA Barcode length (bp) 652
Lane M 1 2 3 4 5 Content DNA ladder Replicate 1 Replicate 2 Replicate 3 Negative control Positive control 700 bp 600 bp M 1 2 3 4 5
Transcribed Image Text:Lane M 1 2 3 4 5 Content DNA ladder Replicate 1 Replicate 2 Replicate 3 Negative control Positive control 700 bp 600 bp M 1 2 3 4 5
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