Calculate the concentration of bacteriophage in the original culture from the following data. Be sure to include units. Show dilution factors for each test tube. Show the final dilution factor for test tube number 4. Show all your math.**Diagram Description:**1. **Original Culture:** - A diagram of a test tube labeled "Original Culture."2. **Test Tube #1:** - Contains 9.9 ml of liquid. - 0.1 ml of the original culture is added.3. **Test Tube #2:** - Contains 9.99 ml of liquid. - 0.01 ml from Test Tube #1 is added.4. **Test Tube #3:** - Contains 9.999 ml of liquid. - 0.001 ml from Test Tube #2 is added.5. **Test Tube #4:** - Contains 9.9 ml of liquid. - 0.1 ml from Test Tube #3 is added.6. **Plating:** - 1.0 ml from Test Tube #4 is plated. - A diagram shows a petri dish with several plaques.**Calculations:**- **Dilution Factor:** - For each transfer, the dilution factor can be calculated by dividing the volume transferred by the total volume in the receiving test tube. - Calculate the cumulative dilution factor for Test Tube #4.- **Concentration of Phage:** - Estimate using the number of plaques observed on the plate.(Spaces are provided below the diagram for the final dilution factor and concentration of phage calculations.) **Note:** Treat as bacterial enumeration. Instead of cfu/ml, use pfu/ml.---*Explanation:* The note emphasizes adjusting the units used for enumeration. While colony-forming units per milliliter (cfu/ml) are typically used for counting bacteria, this text suggests using plaque-forming units per milliliter (pfu/ml) instead. This implies a context where viral enumeration is more appropriate, as pfu/ml is commonly used to quantify viruses.

Name: Calculate the concentration of bacteriophage in the original culture
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Description: Calculate the concentration of bacteriophage in the original culture from the following data. Be sure to include units. Show dilution factors for each test tube. Show the final dilution factor for test tube number 4. Show all your math.**Diagram Des

Virus plaque assay is used to calculate the bacteriophage in a sample.

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Biology

Calculate the concentration of bacteriophage in the original culture from the following data. Be sure to include units. Show dilution factors for each test tube. Show the final dilution factor for test tube number 4. Show all your math. 0.1 ml 0.01 ml 0.001 ml 0.1 ml 1.0 ml plated 9.9 ml 9.99 ml 9.999 ml 9.9 m plaques Original Culture Test Test Test Test Tube #1 Tube #2 Tube #3 Tube #4 Dilution Factor: Concentration of Phage:

Calculate the concentration of bacteriophage in the original culture from the following data. Be sure to include units. Show dilution factors for each test tube. Show the final dilution factor for test tube number 4. Show all your math. 0.1 ml 0.01 ml 0.001 ml 0.1 ml 1.0 ml plated 9.9 ml 9.99 ml 9.999 ml 9.9 m plaques Original Culture Test Test Test Test Tube #1 Tube #2 Tube #3 Tube #4 Dilution Factor: Concentration of Phage:

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Calculate the concentration of bacteriophage in the original culture from the following data. Be sure to include units. Show dilution factors for each test tube. Show the final dilution factor for test tube number 4. Show all your math.

**Diagram Description:**

1. **Original Culture:**
- A diagram of a test tube labeled "Original Culture."

2. **Test Tube #1:**
- Contains 9.9 ml of liquid.
- 0.1 ml of the original culture is added.

3. **Test Tube #2:**
- Contains 9.99 ml of liquid.
- 0.01 ml from Test Tube #1 is added.

4. **Test Tube #3:**
- Contains 9.999 ml of liquid.
- 0.001 ml from Test Tube #2 is added.

5. **Test Tube #4:**
- Contains 9.9 ml of liquid.
- 0.1 ml from Test Tube #3 is added.

6. **Plating:**
- 1.0 ml from Test Tube #4 is plated.
- A diagram shows a petri dish with several plaques.

**Calculations:**

- **Dilution Factor:**
- For each transfer, the dilution factor can be calculated by dividing the volume transferred by the total volume in the receiving test tube.
- Calculate the cumulative dilution factor for Test Tube #4.

- **Concentration of Phage:**
- Estimate using the number of plaques observed on the plate.

(Spaces are provided below the diagram for the final dilution factor and concentration of phage calculations.)

**Note:** Treat as bacterial enumeration. Instead of cfu/ml, use pfu/ml.

---

*Explanation:*

The note emphasizes adjusting the units used for enumeration. While colony-forming units per milliliter (cfu/ml) are typically used for counting bacteria, this text suggests using plaque-forming units per milliliter (pfu/ml) instead. This implies a context where viral enumeration is more appropriate, as pfu/ml is commonly used to quantify viruses.

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