C. Choosing the Proper Buffer Solution 1. Choosing the Proper Buffer Solution In Protein Precipitation, two liters of 5mM buffer solution with pH 5.2 is needed in the isolation of albumin. Which among the following buffer solution is best fitted for said purpose? Justify your answer. Buffer solutions pka Acetate buffer 4.73 Tris- (hydroxymethy) aminomethane 8.08 Phosphate buffer 7.20 Discussion: 2. Preparation of the Chosen Buffer System Calculate and measure the amounts (in grams if solid and in mL if liquid) of weak acid and conjugate base needed to be able to prepare the chosen buffer system in part A above. Express your answer in useful units (that is, prepare it from practical amounts or concentrations of starting materials).
C. Choosing the Proper Buffer Solution 1. Choosing the Proper Buffer Solution In Protein Precipitation, two liters of 5mM buffer solution with pH 5.2 is needed in the isolation of albumin. Which among the following buffer solution is best fitted for said purpose? Justify your answer. Buffer solutions pka Acetate buffer 4.73 Tris- (hydroxymethy) aminomethane 8.08 Phosphate buffer 7.20 Discussion: 2. Preparation of the Chosen Buffer System Calculate and measure the amounts (in grams if solid and in mL if liquid) of weak acid and conjugate base needed to be able to prepare the chosen buffer system in part A above. Express your answer in useful units (that is, prepare it from practical amounts or concentrations of starting materials).
Chapter21: Heparin Infusion Calculations
Section: Chapter Questions
Problem 1.3P
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Transcribed Image Text:C. Choosing the Proper Buffer Solution
1. Choosing the Proper Buffer Solution
In Protein Precipitation, two liters of 5mM buffer solution with pH 5.2 is needed in the
isolation of albumin. Which among the following buffer solution is best fitted for said
purpose? Justify your answer.
Buffer solutions
pKa
Acetate buffer
4.73
Tris- (hydroxymethy) aminomethane
8.08
Phosphate buffer
7.20
Discussion:
2. Preparation of the Chosen Buffer System
Calculate and measure the amounts (in grams if solid and in mL if liquid) of weak acid
and conjugate base needed to be able to prepare the chosen buffer system in part A
above. Express your answer in useful units (that is, prepare it from practical amounts or
concentrations of starting materials).
D. Titration of an Amino acid
Graph: titration of Glutamic acid
Glu
Glu
Glu"
Glu-
COOH
Co0
co0
Co0
H,N*-C -H H,N-C-H H,N*-c-H H,N -C -H
CH2
CH2
CH,
CH2
CH
CH,
CH,
CH2
COOH
соон
co0
Co0
14
12
10
pK
pH
pkg
Isoelectric
point
pk,
1.0
2.0
3.0
Equivalents of COH" added
Owtendaacesoorpary Ganoam ohameey
Tranpency
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