Calculate the specific activity of step#4. Note that percent recovery=% Yield.

Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
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Example of a Protein Purification Scheme:
Purification of the Enzyme Xanthine Dehydrogenase from a Fungus
Volume
Total
Total
Specific
Percent
Fraction
(mL)
Protein (mg) Activity Activity
Recovery
1. Crude extract
2. Salt precipitate
3. Ion-exchange chromatography
|4. Molecular-sieve chromatography
5. Immunoaffinity chromatography
3,800
22,800
2,460
0.108
100
165
2,800
1,190
0.425
48
65
100
720
7.2
29
40
14.5
23
1.8
275
152.108
11
Calculate the specific activity of step#4. Note that percent recovery=% Yield.
Transcribed Image Text:Example of a Protein Purification Scheme: Purification of the Enzyme Xanthine Dehydrogenase from a Fungus Volume Total Total Specific Percent Fraction (mL) Protein (mg) Activity Activity Recovery 1. Crude extract 2. Salt precipitate 3. Ion-exchange chromatography |4. Molecular-sieve chromatography 5. Immunoaffinity chromatography 3,800 22,800 2,460 0.108 100 165 2,800 1,190 0.425 48 65 100 720 7.2 29 40 14.5 23 1.8 275 152.108 11 Calculate the specific activity of step#4. Note that percent recovery=% Yield.
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