BHI TTGTAAAACGACGGCCAGTGAGCGCGCGTAATACGACT M13-20 primer binding site Bsp 1061 goi Primer design worksheet T7 Promoter ...KS primer binding site Primer 17 primer binding site Hind EcoRV EcoRI Pal Eco01091 Xhol Kpn Dra CACTATAGGGCGAATTGGGTACCGGGCCCCCCCTCGAGGTCGAC... Acc Xbal Smal BamHI Spel ...GGTATCGATAAGCTTGATATCGAATTCCTGCAGCCCGGGGGATCCACTAGTTCTAGAGCGGCCGCCACCGCGGTGGAGCTC... SK primer binding site KS primer binding site... Socl Not Eagl Batx I Sac II The diagram above shows a portion of the pSK+ multicloning site. For this worksheet, you need to design primers that could be used to amplify a gene cloned into the EcoRI cloning site. There are 4 primer sites indicated on the diagram. The primers need to be designed to bind to each of the primer binding sites indicated on the diagram. Fill in the following information: Identify all possible Forward primers and write out their sequence: 5'-3' sequence
BHI TTGTAAAACGACGGCCAGTGAGCGCGCGTAATACGACT M13-20 primer binding site Bsp 1061 goi Primer design worksheet T7 Promoter ...KS primer binding site Primer 17 primer binding site Hind EcoRV EcoRI Pal Eco01091 Xhol Kpn Dra CACTATAGGGCGAATTGGGTACCGGGCCCCCCCTCGAGGTCGAC... Acc Xbal Smal BamHI Spel ...GGTATCGATAAGCTTGATATCGAATTCCTGCAGCCCGGGGGATCCACTAGTTCTAGAGCGGCCGCCACCGCGGTGGAGCTC... SK primer binding site KS primer binding site... Socl Not Eagl Batx I Sac II The diagram above shows a portion of the pSK+ multicloning site. For this worksheet, you need to design primers that could be used to amplify a gene cloned into the EcoRI cloning site. There are 4 primer sites indicated on the diagram. The primers need to be designed to bind to each of the primer binding sites indicated on the diagram. Fill in the following information: Identify all possible Forward primers and write out their sequence: 5'-3' sequence
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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![Best II
TTGTAAAACGACGGCCAGTGAGCGCGCGTAATACGACT
M13-20 primer binding site
Primer design worksheet
T7 Promoter
Bsp 1061
Cial
...KS primer binding site
Kpn 1
Eco01091
Dra II
CACTATAGGGCGAATTGGGTACCGGGCCCCCCCTCGAGGTCGAC..
17 primer binding site
Not I
Hind II EcoRV EcoRI Pst l Smal BamHI Spel Xbal | Eagl BstX | Sac II
T
...GGTATCGATAAGCTTGATATCGAATTCCTGCAGCCCGGGGGATCCACTAGTTCTAGAGCGGCCGCCACCGCGGTGGAGCTC...
SK primer binding site
Primer
Xhol
Identify all possible Reverse primers and write out their sequence
KS primer binding site...
The diagram above shows a portion of the pSK+ multicloning site. For this worksheet, you need
to design primers that could be used to amplify a gene cloned into the EcoRI cloning site.
There are 4 primer sites indicated on the diagram. The primers need to be designed to bind to
each of the primer binding sites indicated on the diagram.
Fill in the following information:
Identify all possible Forward primers and write out their sequence:
Primer
5'-3' sequence
5'-3' sequence
Şac 1](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2Fdb3dca1d-57a3-4839-be8e-2a543baf6bdb%2F785f941b-f718-4a94-9eaa-976cdc794f35%2F79unhu7_processed.png&w=3840&q=75)
Transcribed Image Text:Best II
TTGTAAAACGACGGCCAGTGAGCGCGCGTAATACGACT
M13-20 primer binding site
Primer design worksheet
T7 Promoter
Bsp 1061
Cial
...KS primer binding site
Kpn 1
Eco01091
Dra II
CACTATAGGGCGAATTGGGTACCGGGCCCCCCCTCGAGGTCGAC..
17 primer binding site
Not I
Hind II EcoRV EcoRI Pst l Smal BamHI Spel Xbal | Eagl BstX | Sac II
T
...GGTATCGATAAGCTTGATATCGAATTCCTGCAGCCCGGGGGATCCACTAGTTCTAGAGCGGCCGCCACCGCGGTGGAGCTC...
SK primer binding site
Primer
Xhol
Identify all possible Reverse primers and write out their sequence
KS primer binding site...
The diagram above shows a portion of the pSK+ multicloning site. For this worksheet, you need
to design primers that could be used to amplify a gene cloned into the EcoRI cloning site.
There are 4 primer sites indicated on the diagram. The primers need to be designed to bind to
each of the primer binding sites indicated on the diagram.
Fill in the following information:
Identify all possible Forward primers and write out their sequence:
Primer
5'-3' sequence
5'-3' sequence
Şac 1
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