**Primer Design Worksheet**The diagram above shows a portion of the pSK+ multicloning site. For this worksheet, you need to design primers that could be used to amplify a gene cloned into the **EcoRI** cloning site.There are 4 primer sites indicated on the diagram. The primers need to be designed to bind to each of the primer binding sites indicated on the diagram.**Fill in the following information:****Identify all possible Forward primers and write out their sequence:**- **Primer** 5’-3’ sequence **Identify all possible Reverse primers and write out their sequence:**- **Primer** 5’-3’ sequence---**Diagram Explanation:**- The graphic shows a DNA sequence with labeled regions including restriction enzyme sites (e.g., **BssH II**, **Nhe I**, **EcoRI**), a **T7 Promoter**, and primer binding sites (**M13 -20**, **T7**, **KS**, **SK**).- The sequence is labeled at several restriction sites with their enzyme names, illustrating where cuts can be made for gene cloning.- Arrows indicate the orientation of the **T7 promoter** and primer binding sites, showing which direction the primers should be designed to bind.- The goal is to use this map to determine forward and reverse primers for amplifying the gene at the EcoRI site.

Name: **Primer Design Worksheet**The diagram above shows a portion of the p
Uploaded: 2024-03-20T06:42:55.000Z
Channel: Bartleby
Description: **Primer Design Worksheet**The diagram above shows a portion of the pSK+ multicloning site. For this worksheet, you need to design primers that could be used to amplify a gene cloned into the **EcoRI** cloning site.There are 4 primer sites indicated