Above shows the 4 disulfide bonds formed in the molecule RNAse A (shown as green connectors), famously studied by Christian Anfinson. Also shown is a graph of temperature (X-axis) vs. fraction unfolded (Y-axis) of the wild type protein and cysteine to alanine mutations that separately eliminate each of the disulfide pairs. Choose the 3 statements below that are TRUE.
Above shows the 4 disulfide bonds formed in the molecule RNAse A (shown as green connectors), famously studied by Christian Anfinson. Also shown is a graph of temperature (X-axis) vs. fraction unfolded (Y-axis) of the wild type protein and cysteine to alanine mutations that separately eliminate each of the disulfide pairs. Choose the 3 statements below that are TRUE.
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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Transcribed Image Text:40-95
A
Lys41
His119
His12
84-26
110-58
72-65
В
N
58
65 72
C
110
40
95
A 1.0
0.8
58
40
1104 95
Wild-
type
0.6
26
65
84
72
0.4
0.2
0.0
Above shows the 4 disulfide bonds formed in the molecule RNASE A (shown as green connectors),
famously studied by Christian Anfinson. Also shown is a graph of temperature (X-axis) vs. fraction
unfolded (Y-axis) of the wild type protein and cysteine to alanine mutations that separately eliminate
each of the disulfide pairs. Choose the 3 statements below that are TRUE.
Because each mutation affects thermodynamic stability, each is also likely to affect RNAse function.
Cys to Ala mutations in this context would be considered non-conservative.
Given the data, treatment of the wildtype enzyme with beta-mercaptoethanol would likely shift the Tm
to the right.
The disulfide bond that least affects stability encloses the smallest sequence loop.
O According to the data, mutations affect both the Tm and the cooperativity of melting.
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