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Q: Draw a schematic diagram showing the components involved in the regulation of the tryptophan оperon.
A: Answer
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Briefly explain about the upstream processing of Baker's yeast
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Solved in 2 steps
- What are the results in the extraction of invertase from baker's yeast? (Lab purposes)Yarrowia lipolytica(Yl) is a non-conventional yeast that diverged from baker’s yeast, Saccharomyces cerevisiae(Sc), early in evolutionary history. The regulation of glycolytic enzymes in Yl differs from that of Sc. The data below pertain to regulation of phosphofructokinase 1 (PFK). Data for Yl is shown with black circles and data for Sc is shown with white circles. Pfk for both species is a homo-octomer (eight identical subunits). Given the data below, which species is more likely to be susceptible to allosteric modification? Briefly explain.Yarrowia lipolytica(Yl) is a non-conventional yeast that diverged from baker’s yeast, Saccharomyces cerevisiae(Sc), early in evolutionary history. The regulation of glycolytic enzymes in Yl differs from that of Sc. The data below pertain to regulation of phosphofructokinase 1 (PFK). Data for Yl is shown with black circles and data for Sc is shown with white circles. Imagine that the genes for phosphofructose kinase 2 and fructose 2,6-bisphosphatase were deleted in each species. Which species would you expect to have greater metabolic disruption and why?
- Give the schematic representation of an overall view of Krebs’ cycle.Describe results that could be obtained from ribosomeprofiling that would indicate the existence of aregulatory mechanism operating at the level oftranslational initiation.Sydney Brennen isolated Salmonella typhimurium mutants that were implicated in the biosynthesis of tryptophan and would not grow on minimal medium supplemented with intermediates in tryptophan biosynthesis, some mutants were able to grow while others remained unable to grow. Review the data attached to order the biosynthetic pathway by both enzymatic step and by intermediate biomolecule. Label the step impacted by each of the mutant cell lines.
- What is role of yeast alcohol dehydrogenase in cellular and organismal mechanisms.Part 2A: Preparation of solutions necessary for the investigation of transmembrane transport in yeast In Part 2A, you will make the solutions necessary for Part 2B in the following lab session. 56 mM glucose (Fisher Chemical Company, Waltham, MA) 20 mM HEPES (Fisher Chemical Company, Waltham, MA) pH 6.8 Store this at 4 degrees until the day of the experiment. 03. Determine the total amount of YGM solution you'll need next week in Part 1B, and add to that a good amount extra to be prepared for repeats. Describe in your lab notebook how much YGM your group will likely need AND your exact plan for making it. Tip: Work through the procedure, find where you need YGM and take note of how many experiments you will be doing!Why is penicillin such an effective inhibitor of the transpeptidase?
- Researchers isolated a yeast phosphofructokinase (PFK) mutant in which a serine at the fructose-2,6-bisphosphate binding site was replaced with an aspartate residue. The amino acid substitution completely abolished the binding of fructose-2,6-bisphosphate to PFK. There was a dramatic decline in glucose consumption and ethanol production in the mutant compared to control yeast. Why can't the mutant PFK bind with fructose-2,6-bisphosphate?Describe a common feature at the active site of serine proteases and acetyl cholinesteraseWhat are the structural elements that are required for proper function in yeast alcohol dehydrogenase ADH1?